Interrogation of gossypol therapy in glioblastoma implementing cell line and patient-derived tumour models
| Autor: | Patrick Dicker, Cherry Gao, Jochen H. M. Prehn, Benjamin E. Rich, Jian Wang, Liam Shiels, Monika A. Jarzabek, Keith L. Ligon, John J. Callanan, V Amberger-Murphy, Agnieszka Zagozdzon, William M. Gallagher, Annette T. Byrne |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Cancer Research
Pathology medicine.medical_specialty Angiogenesis GBM 03 medical and health sciences angiogenesis Mice 0302 clinical medicine In vivo Cell Line Tumor medicine Animals Humans Viability assay 030304 developmental biology Tube formation 0303 health sciences Mice Inbred BALB C Temozolomide business.industry Brain Neoplasms apoptosis Gossypol Cancer medicine.disease invasion Xenograft Model Antitumor Assays 3. Good health Endothelial stem cell Oncology Apoptosis 030220 oncology & carcinogenesis Cancer research Female business Translational Therapeutics TMZ Glioblastoma medicine.drug |
| Zdroj: | British Journal of Cancer ResearcherID |
| ISSN: | 1532-1827 0007-0920 |
| Popis: | Background: Glioblastoma (GBM), being a highly vascularised and locally invasive tumour, is an attractive target for anti-angiogenic and anti-invasive therapies. The GBM/endothelial cell response to gossypol/temozolomide (TMZ) treatment was investigated with a particular aim to assess treatment effects on cancer hallmarks. Methods: Cell viability, endothelial tube formation and GBM tumour cell invasion were variously assessed following combined treatment in vitro. The U87MG-luc2 subcutaneous xenograft model was used to investigate therapeutic response in vivo. Viable tumour response to treatment was interrogated using immunohistochemistry. Combined treatment protocols were also tested in primary GBM patient-derived cultures. Results: An endothelial/GBM cell viability inhibitory effect, as well as an anti-angiogenic and anti-invasive response, to combined treatment have been demonstrated in vitro. A significantly greater anti-proliferative (P=0.020, P=0.030), anti-angiogenic (P=0.040, P |
| Databáze: | OpenAIRE |
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