Variability of essential and non-essential trace elements in the follicular fluid of women undergoing in vitro fertilization (IVF)

Autor: Alexandra McGough, Beth J. Feingold, Victor Y. Fujimoto, Patrick J. Parsons, Richard W. Browne, Nikolaus Lenhart, Recai Yucel, Michael S. Bloom, Aubrey L. Galusha, Celeste D. Butts, Rebecca Wong, Evelyn Mok-Lin
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Future studies
Health
Toxicology and Mutagenesis
medicine.medical_treatment
0211 other engineering and technologies
Reproductive health and childbirth
02 engineering and technology
Urine
010501 environmental sciences
Medical and Health Sciences
01 natural sciences
Environmental pollution
Ovarian Follicle
In vitro fertilization
Follicular phase
GE1-350
General Medicine
Reliability
Pollution
TD172-193.5
Biomarker (medicine)
Female
Adult
Fertilization in Vitro
Follicular fluid
Biology
Article
Andrology
Clinical Research
medicine
Humans
Strategic
Nutrition
0105 earth and related environmental sciences
021110 strategic
defence & security studies
Trace elements
In vitro fertilisation
Biologic variability
Contraception/Reproduction
Prevention
Public Health
Environmental and Occupational Health
Trace element
Reproducibility of Results
Mercury
Defence & Security Studies
Environmental sciences
Infertility
Chemical Sciences
Oocytes
Body mass index
Environmental Sciences
Biomarkers
Zdroj: Ecotoxicology and Environmental Safety, Vol 209, Iss, Pp 111733-(2021)
Ecotoxicol Environ Saf
ISSN: 0147-6513
Popis: Both essential and non-essential elements have been associated with female reproductive function in epidemiologic investigations, including among IVF populations. To date, most investigators have used blood or urine to assess biomarkers of exposure, with few employing ovarian follicular fluid (FF). FF may offer a more direct "snapshot" of the oocyte microenvironment than blood or urine, however previous studies report follicle-to-follicle variability in FF constituents that may contribute to exposure misclassification. Our objectives were to investigate sources of trace element variability, to estimate FF biomarker reliability among women undergoing IVF (n=34), and to determine the minimum number of follicles required to estimate subject-specific mean concentrations. We measured As, Hg, Cd, Pb, Cu, Mn, Se, and Zn in FF samples using inductively coupled plasma tandem mass spectrometry. Inter-subject (between-women) variability contributed most of the variability in FF element concentrations, with ovarian, follicular, and analytical as smaller sources of variability. The proportion of variability attributable to sources between-follicles differed by age, body mass index (BMI), race, and cigarette smoking for Cu, Se, and Zn, by BMI and cigarette smoking for As, by primary infertility diagnosis for Hg, Cu, Se, and Zn, and by ovarian stimulation protocol for Mn and Se. Four to five individual follicles were sufficient to estimate subject-specific mean Cu, Se, and Zn concentrations, while >14 were necessary for As, Hg, Cd, Pb, and Mn. Overall, our results suggest that FF is a suitable source of biomarkers of As and Hg exposure in ovarian follicles. Although limited in size, our study offers the most comprehensive exploration of biological variation in FF trace elements to date and may provide guidance for future studies of ovarian trace element exposures.
Databáze: OpenAIRE
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