Optimization of GOPS-Based Functionalization Process and Impact of Aptamer Grafting on the Si Nanonet FET Electrical Properties as First Steps towards Thrombin Electrical Detection
| Autor: | Céline Ternon, Valérie Stambouli, Fanny Morisot, Ganesh Jayakumar, Nicolas Spinelli, Mireille Mouis, Christoforos G. Theodorou, Laetitia Rapenne, Per-Erik Hellström, X. Mescot, Bassem Salem, Monica Vallejo-Perez |
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| Přispěvatelé: | Laboratoire des matériaux et du génie physique (LMGP ), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP ), Université Grenoble Alpes (UGA)-Université Grenoble Alpes (UGA), Commissariat à l'énergie atomique et aux énergies alternatives - Laboratoire d'Electronique et de Technologie de l'Information (CEA-LETI), Direction de Recherche Technologique (CEA) (DRT (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Institut de Microélectronique, Electromagnétisme et Photonique - Laboratoire d'Hyperfréquences et Caractérisation (IMEP-LAHC), Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP ), Université Grenoble Alpes (UGA), Royal Institute of Technology [Stockholm] (KTH ), Laboratoire des technologies de la microélectronique (LTM ), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA), ANR-16-PILO-0003,Convergence,Frictionless Energy Efficient Convergent Wearables For Healthcare and Lifestyle Applications(2016), ANR-10-LABX-0055,MINOS Lab,Minatec Novel Devices Scaling Laboratory(2010), European Project: 688329,H2020,H2020-ICT-2015,Nanonets2Sense(2016) |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Materials science
GOPS-based functionalization General Chemical Engineering Aptamer Nanowire nanonet Nanotechnology 02 engineering and technology 010402 general chemistry 01 natural sciences Article lcsh:Chemistry Thrombin Scanning transmission electron microscopy Fluorescence microscope medicine field effect transistors General Materials Science [SPI.NANO]Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics UV-assisted functionalization ComputingMilieux_MISCELLANEOUS 021001 nanoscience & nanotechnology Grafting 0104 chemical sciences silicon nanowires lcsh:QD1-999 Surface modification Field-effect transistor 0210 nano-technology aptamer grafting medicine.drug |
| Zdroj: | Nanomaterials Nanomaterials, MDPI, 2020, 10 (9), pp.1842. ⟨10.3390/nano10091842⟩ Nanomaterials, 2020, 10 (9), pp.1842. ⟨10.3390/nano10091842⟩ Nanomaterials, Vol 10, Iss 1842, p 1842 (2020) Volume 10 Issue 9 |
| ISSN: | 2079-4991 |
| DOI: | 10.3390/nano10091842⟩ |
| Popis: | Field effect transistors (FETs) based on networks of randomly oriented Si nanowires (Si nanonets or Si NNs) were biomodified using Thrombin Binding Aptamer (TBA&ndash 15) probe with the final objective to sense thrombin by electrical detection. In this work, the impact of the biomodification on the electrical properties of the Si NN&ndash FETs was studied. First, the results that were obtained for the optimization of the (3-Glycidyloxypropyl)trimethoxysilane (GOPS)-based biofunctionalization process by using UV radiation are reported. The biofunctionalized devices were analyzed by atomic force microscopy (AFM) and scanning transmission electron microscopy (STEM), proving that TBA&ndash 15 probes were properly grafted on the surface of the devices, and by means of epifluorescence microscopy it was possible to demonstrate that the UV-assisted GOPS-based functionalization notably improves the homogeneity of the surface DNA distribution. Later, the electrical characteristics of 80 devices were analyzed before and after the biofunctionalization process, indicating that the results are highly dependent on the experimental protocol. We found that the TBA&ndash 15 hybridization capacity with its complementary strand is time dependent and that the transfer characteristics of the Si NN&ndash FETs obtained after the TBA&ndash 15 probe grafting are also time dependent. These results help to elucidate and define the experimental precautions that must be taken into account to fabricate reproducible devices. |
| Databáze: | OpenAIRE |
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