Nonsense-Mediated mRNA Decay in Yeast Does Not Require PAB1 or a Poly(A) Tail
| Autor: | Stacie Meaux, Ambro van Hoof, Kristian E. Baker |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Saccharomyces cerevisiae Proteins
Polyadenylation RNA Stability Recombinant Fusion Proteins Green Fluorescent Proteins Nonsense-mediated decay Saccharomyces cerevisiae Biology Poly(A)-Binding Proteins Article Galactokinase Open Reading Frames 03 medical and health sciences Genes Reporter P-bodies Poly(A)-binding protein Genes Synthetic RNA Catalytic RNA Messenger Molecular Biology 030304 developmental biology 0303 health sciences Messenger RNA 030302 biochemistry & molecular biology Translation (biology) Cell Biology Molecular biology Stop codon Open reading frame Codon Nonsense biology.protein |
| Zdroj: | Molecular Cell. 29:134-140 |
| ISSN: | 1097-2765 |
| Popis: | Eukaryotic mRNAs harboring premature translation termination codons are recognized and rapidly degraded by the nonsense-mediated mRNA decay (NMD) pathway. The mechanism for discriminating between mRNAs that terminate translation prematurely and those subject to termination at natural stop codons remains unclear. Studies in multiple organisms indicate that proximity of the termination codon to the 3' poly(A) tail and the poly(A) RNA-binding protein, PAB1, constitute the critical determinant in NMD substrate recognition. We demonstrate that mRNA in yeast lacking a poly(A) tail can be destabilized by introduction of a premature termination codon and, importantly, that this mRNA is a substrate of the NMD machinery. We further show that, in cells lacking Pab1p, mRNA substrate recognition and destabilization by NMD are intact. These results establish that neither the poly(A) tail nor PAB1 is required in yeast for discrimination of nonsense-codon-containing mRNA from normal by NMD. |
| Databáze: | OpenAIRE |
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