Transient expression of clusterin (sulfated glycoprotein-2) during development of rat pancreas
| Autor: | Chun Bg, M Bendayan, Jeong Sy, Kang Sw, Foster Dn, Bon Hong Min, In Sun Park, Crabo Bg |
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| Rok vydání: | 1998 |
| Předmět: |
medicine.medical_specialty
Endocrinology Diabetes and Metabolism Blotting Western Immunocytochemistry Gestational Age Kidney Rats Sprague-Dawley Endocrinology Western blot Internal medicine Gene expression medicine Animals Northern blot Lung Pancreas Glycoproteins Brain Chemistry Complement Inactivator Proteins Clusterin biology medicine.diagnostic_test Myocardium Embryogenesis Brain Heart Embryo Blotting Northern Immunohistochemistry eye diseases Rats medicine.anatomical_structure Liver biology.protein sense organs Molecular Chaperones |
| Zdroj: | Journal of Endocrinology. 158:43-52 |
| ISSN: | 1479-6805 0022-0795 |
| Popis: | Clusterin has been known to play important roles not only in remodeling damaged tissues, but also in tissue reorganization during embryonic development. In the present study, we have investigated the expression of clusterin in the endocrine pancreas during embryonic development. Although a weak immunoreaction was detected in some pancreatic primordial cells at day 14 of gestation, distinct clusterin expression was identified by immunocytochemistry and Northern blot analysis at the 16th day of gestation. Clusterin-producing cells, which corresponded to insulin-containing cells, accounted for the major portion of the developing islet of Langerhans up to 18 days of gestation. Thereafter, clusterin-producing cells display similar distribution and morphological features to glucagon-producing cells. Clusterin expressed in the pancreas was shown by Western blot analysis to be a disulfide-linked heterodimer of 70 kDa with an alpha-subunit of 32 kDa. During early developmental stages, however, we found that proteolytic internal cleavage of the clusterin molecule occurred from the 18th day of gestation. Only one 70 kDa band on the 16th day and two bands (32 kDa and 70 kDa) on the 18th day of gestation were detected by Western blot analysis even in reducing conditions, while only a single 32 kDa band was detected on the second day after birth. The levels of clusterin mRNA in the pancreas transiently increased from the 16th day of gestation to the second day after birth, during the period when active cellular reorganization takes place to form the classic cellular features of the islet. Among various tissue (kidney, brain, liver, heart, lung and pancreas) the levels of clusterin mRNA were the highest in the pancreas from the 18th day of gestation to the second day after birth. In contrast, the lowest expression was observed in adult pancreatic tissue. The higher expression of clusterin in developing pancreas must indicate its involvement in tissue organization during development. |
| Databáze: | OpenAIRE |
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