Vacuolar-type H+-ATPases at the plasma membrane regulate pH and cell migration in microvascular endothelial cells
| Autor: | Jose D. Rojas, David E. Wesson, Gloria M. Martinez, Karina Bakunts, Cynthia J. Meininger, M. Reuveni, Mary J.C. Hendrix, Souad R. Sennoune, Elisabeth A. Seftor, Raul Martinez-Zaguilan, Debasish Maiti, Shankar C. Sanka, Guoyao Wu |
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| Rok vydání: | 2006 |
| Předmět: |
Vacuolar Proton-Translocating ATPases
Sodium-Hydrogen Exchangers Endothelium Physiology Angiogenesis ATPase Biology chemistry.chemical_compound Cell Movement Physiology (medical) medicine Animals Rats Inbred BB Cells Cultured Microcirculation Cell Membrane Bafilomycin Cell migration Hydrogen-Ion Concentration Immunohistochemistry Rats Cell biology Sodium–hydrogen antiporter Membrane medicine.anatomical_structure chemistry biology.protein Endothelium Vascular Cardiology and Cardiovascular Medicine Intracellular |
| Zdroj: | American Journal of Physiology-Heart and Circulatory Physiology. 291:H1147-H1157 |
| ISSN: | 1522-1539 0363-6135 |
| DOI: | 10.1152/ajpheart.00166.2006 |
| Popis: | Microvascular endothelial cells involved in angiogenesis are exposed to an acidic environment that is not conducive for growth and survival. These cells must exhibit a dynamic intracellular (cytosolic) pH (pHcyt) regulatory mechanism to cope with acidosis, in addition to the ubiquitous Na+/H+exchanger and HCO3−-based H+-transporting systems. We hypothesize that the presence of plasmalemmal vacuolar-type proton ATPases (pmV-ATPases) allows microvascular endothelial cells to better cope with this acidic environment and that pmV-ATPases are required for cell migration. This study indicates that microvascular endothelial cells, which are more migratory than macrovascular endothelial cells, express pmV-ATPases. Spectral imaging microscopy indicates a more alkaline pHcytat the leading than at the lagging edge of microvascular endothelial cells. Treatment of microvascular endothelial cells with V-ATPase inhibitors decreases the proton fluxes via pmV-ATPases and cell migration. These data suggest that pmV-ATPases are essential for pHcytregulation and cell migration in microvascular endothelial cells. |
| Databáze: | OpenAIRE |
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