Spermidine regulatesVibrio choleraebiofilm formation via transport and signaling pathways
| Autor: | Ece Karatan, C. P. Cartaya‐Marin, Zachary M. Parker, Marcus W. McGinnis, Nicholas Emmanuel Walter, Alex C. Rutkovsky |
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| Rok vydání: | 2009 |
| Předmět: |
Spermidine transport
Spermidine Biology medicine.disease_cause Microbiology chemistry.chemical_compound Bacterial Proteins Genetics medicine Humans Vibrio cholerae Molecular Biology Polyamine transport Norspermidine Biofilm Membrane Transport Proteins Biological Transport Periplasmic space biochemical phenomena metabolism and nutrition chemistry Biofilms Polyamine Gene Deletion Signal Transduction |
| Zdroj: | FEMS Microbiology Letters. 299:166-174 |
| ISSN: | 1574-6968 0378-1097 |
| Popis: | Vibrio cholerae, the causative agent of the devastating diarrheal disease cholera, can form biofilms on diverse biotic and abiotic surfaces. Biofilm formation is important for the survival of this organism both in its natural environment and in the human host. Development of V. cholerae biofilms are regulated by complex regulatory networks that respond to environmental signals. One of these signals, norspermidine, is a polyamine that enhances biofilm formation via the NspS/MbaA signaling system. In this work, we have investigated the role of the polyamine spermidine in regulating biofilm formation in V. cholerae. We show that spermidine import requires PotD1, an ortholog of the periplasmic substrate-binding protein of the spermidine transport system in Escherichia coli. We also show that deletion of the potD1 gene results in a significant increase in biofilm formation. We hypothesize that spermidine imported into the cell hinders biofilm formation. Exogenous spermidine further reduces biofilm formation in a PotD1-independent, but NspS/MbaA-dependent, manner. Our results suggest that polyamines affect biofilm formation in V. cholerae via multiple pathways involving both transport and signaling networks. |
| Databáze: | OpenAIRE |
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