Impact of simulated Martian conditions on (facultatively) anaerobic bacterial strains from different Mars analogue sites
| Autor: | Beblo-Vranesevic, Kristina, Bohmeier, Maria, Schleumer, Sven, Rabbow, Elke, Perras, Alexandra K., Moissl-Eichinger, Christine, Schwendner, Petra, Cockell, Charles S., Vannier, Pauline, Marteinsson, Viggo T., Monaghan, Euan P., Riedo, Andreas, Ehrenfreund, Pascale, Garcia-Descalzo, Laura, Gómez, Felipe, Malki, Moustafa, Amils, Ricardo, Gaboyer, Frédéric, Hickman-Lewis, Keyron, Westall, Frances, Cabezas, Patricia, Walter, Nicolas, Rettberg, Petra |
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| Přispěvatelé: | Deutsches Zentrum für Luft- und Raumfahrt [Köln] (DLR), DLR Institut für Luft- und Raumfahrtmedizin, Medical University Graz, UK Centre for Astrobiology, SUPA School of Physics and Astronomy [Edinburgh], University of Edinburgh-University of Edinburgh, Laboratoire de microbiologie des environnements extrêmophiles (LM2E), Centre National de la Recherche Scientifique (CNRS)-Université de Brest (UBO)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), MATIS - Prokaria, Leiden Observatory [Leiden], Universiteit Leiden [Leiden], Centro de Astrobiologia [Madrid] (CAB), Instituto Nacional de Técnica Aeroespacial (INTA)-Consejo Superior de Investigaciones Científicas [Madrid] (CSIC), Centro de Biología Molecular Severo Ochoa [Madrid] (CBMSO), Universidad Autonoma de Madrid (UAM)-Consejo Superior de Investigaciones Científicas [Madrid] (CSIC), Centre de biophysique moléculaire (CBM), Université d'Orléans (UO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), European Science Foundation (ESF), Rettberg, P. [0000-0003-4439-2395], García Descalzo, L. [0000-0002-0083-6786], Cabezas, P. [0000-0002-6336-4093], Marteinsson, V. [0000-0001-8340-821X], Gómez, F. [0000-0001-9977-7060], Antunes, André |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Time Factors
Extraterrestrial Environment Cell Survival Firmicutes Mars Anaerobic Bacterial Radiation Tolerance Bacteria Anaerobic Strahlenbiologie Enterobacteriaceae Stress Physiological MASE Carnobacteriaceae Desiccation Clostridium Perchlorates simulated Martian conditions Martian conditions anearobic bacterial strains Sodium Compounds Yersinia Oxidative Stress 13. Climate action [SDU]Sciences of the Universe [physics] Simulated Extreme Environments |
| Zdroj: | Current Issues in Molecular Biology, 38, 103-122 Current Issues in Molecular Biology Current Issues in Molecular Biology, Horizon Scientific Press, 2020, 38, pp.103-122. ⟨10.21775/cimb.038.103⟩ DIGITAL.INTA Repositorio Digital del Instituto Nacional de Técnica Aeroespacial Instituto Nacional de Técnica Aeroespacial (INTA) instname Digital.CSIC. Repositorio Institucional del CSIC |
| ISSN: | 1467-3037 |
| DOI: | 10.21775/cimb.038.103⟩ |
| Popis: | Five bacterial (facultatively) anaerobic strains, namely Buttiauxella sp. MASE-IM-9, Clostridium sp. MASE-IM-4, Halanaerobium sp. MASE-BB-1, Trichococcus sp. MASE-IM-5, and Yersinia intermedia MASE-LG-1 isolated from different extreme natural environments were subjected to Mars relevant environmental stress factors in the laboratory under controlled conditions. These stress factors encompassed low water activity, oxidizing compounds, and ionizing radiation. Stress tests were performed under permanently anoxic conditions. The survival rate after addition of sodium perchlorate (Na-perchlorate) was found to be species-specific. The inter-comparison of the five microorganisms revealed that Clostridium sp. MASE-IM-4 was the most sensitive strain (D10-value (15 min, NaClO4) = 0.6 M). The most tolerant microorganism was Trichococcus sp. MASE-IM-5 with a calculated D10-value (15 min, NaClO4) of 1.9 M. Cultivation in the presence of Na-perchlorate in Martian relevant concentrations up to 1 wt% led to the observation of chains of cells in all strains. Exposure to Na-perchlorate led to a lowering of the survival rate after desiccation. Consecutive exposure to desiccating conditions and ionizing radiation led to additive effects. Moreover, in a desiccated state, an enhanced radiation tolerance could be observed for the strains Clostridium sp. MASE-IM-4 and Trichococcus sp. MASE-IM-5. These data show that anaerobic microorganisms from Mars analogue environments can resist a variety of Martian-simulated stresses either individually or in combination. However, responses were species-specific and some Mars-simulated extremes killed certain organisms. Thus, although Martian stresses would be expected to act differentially on microorganisms, none of the expected extremes tested here and found on Mars prevent the growth of anaerobic microorganisms. With funding from the Spanish government through the "María de Maeztu Unit of Excellence" accreditation (MDM-2017-0737) |
| Databáze: | OpenAIRE |
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