Facile Microwave Assisted Synthesis of Silver Nanostars for Ultrasensitive Detection of Biological Analytes by SERS
| Autor: | Radu Nicolae Revnic, Gabriela Fabiola Știufiuc, Valentin Toma, Anca Onaciu, Alin Moldovan, Adrian Bogdan Țigu, Eva Fischer-Fodor, Romulus Tetean, Emil Burzo, Rareș Ionuț Știufiuc |
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| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
Silver
anisotropic silver nanostars SERS bioanalytes cell lysates pharmaceutical compounds Organic Chemistry Water General Medicine Microscopy Atomic Force Spectrum Analysis Raman Catalysis Computer Science Applications Inorganic Chemistry Physical and Theoretical Chemistry Microwaves Molecular Biology Spectroscopy |
| Zdroj: | International Journal of Molecular Sciences; Volume 23; Issue 15; Pages: 8830 |
| ISSN: | 1422-0067 |
| DOI: | 10.3390/ijms23158830 |
| Popis: | We report a very simple, rapid and reproducible method for the fabrication of anisotropic silver nanostars (AgNS) that can be successfully used as highly efficient SERS substrates for different bioanalytes, even in the case of a near-infra-red (NIR) excitation laser. The nanostars have been synthesized using the chemical reduction of Ag+ ions by trisodium citrate. This is the first research reporting the synthesis of AgNS using only trisodium citrate as a reducing and stabilizing agent. The key elements of this original synthesis procedure are rapid hydrothermal synthesis of silver nanostars followed by a cooling down procedure by immersion in a water bath. The synthesis was performed in a sealed bottom flask homogenously heated and brought to a boil in a microwave oven. After 60 s, the colloidal solution was cooled down to room temperature by immersion in a water bath at 35 °C. The as-synthesized AgNS were washed by centrifugation and used for SERS analysis of test molecules (methylene blue) as well as biological analytes: pharmaceutical compounds with various Raman cross sections (doxorubicin, atenolol & metoprolol), cell lysates and amino acids (methionine & cysteine). UV-Vis absorption spectroscopy, (Scanning) Transmission Electron Microscopy ((S)TEM) and Atomic Force Microscopy (AFM) have been employed for investigating nanostars’ physical properties. |
| Databáze: | OpenAIRE |
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