Composition of sulfated glycosaminoglycans and immunodistribution of chondroitin sulfate in deeply infiltrating endometriosis affecting the rectosigmoid
| Autor: | Maisa L.S. Souza, Mauricio Simões Abrão, Luiz Eurico Nasciutti, Daniel Escorsim Machado, Plínio Tostes Berardo, Luiz-Claudio F. Silva |
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| Rok vydání: | 2009 |
| Předmět: |
Adult
Endometriosis Dermatan Sulfate General Physics and Astronomy Dermatan sulfate Glycosaminoglycan chemistry.chemical_compound Sulfation Structural Biology Humans General Materials Science Chondroitin sulfate Glycosaminoglycans Histocytochemistry Chondroitin Sulfates Metachromasia Cell Biology Heparan sulfate Middle Aged Immunohistochemistry Molecular biology Staining chemistry Biochemistry Female Heparitin Sulfate Immunostaining |
| Zdroj: | Micron. 40:639-645 |
| ISSN: | 0968-4328 |
| DOI: | 10.1016/j.micron.2009.02.005 |
| Popis: | The composition of sulfated glycosaminoglycans (GAGs) and the tissue distribution of chondroitin sulfate (CS) were analyzed in deeply infiltrating endometriosis (DIE) of rectosigmoid, using metachromatic staining, and biochemical analysis employing electrophoresis before and after specific enzymatic or chemical degradations, and immunostaining with an antibody against CS. The sulfated GAGs were characterized as dermatan sulfate (DS), heparan sulfate (HS) and CS; and DS strongly predominated compared to HS and CS. Immunostaining procedures showed that CS was concentrated in the endometriosis foci, distributed throughout the stroma around the glands. This is the first report describing the composition of sulfated GAGs and the tissue location of CS in DIE by means of histochemical, biochemical and immunohistochemical analyses. These results confirmed that in DIE of rectosigmoid, as in eutopic endometrium [Nasciutti, L.E., Ferrari, R., Berardo, P.T., Souza, M.L.S., Takiya, C.M., Borojevic, R., Abrao, M.S., Silva, L.C.F., 2006. Distribution of chondroitin sulfate in human endometrium. Micron 37, 544–550], CS was the dominant sulfated GAG in stroma of the lesion foci. |
| Databáze: | OpenAIRE |
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