Mitogen-activated protein kinase regulates FSH-induced expression of tissue-type plasminogen activator through an activator protein 1 response element
| Autor: | Tao Liu, Yi-Xun Liu, Zhao-Yuan Hu, Fu-Qing Yu, Cui-Ling Lu, Wei Yang |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
MAPK/ERK pathway
endocrine system Time Factors Endocrinology Diabetes and Metabolism p38 mitogen-activated protein kinases Response Elements p38 Mitogen-Activated Protein Kinases Rats Sprague-Dawley Endocrinology Animals Protein kinase A Extracellular Signal-Regulated MAP Kinases Cells Cultured Mitogen-Activated Protein Kinase 1 Granulosa Cells Mitogen-Activated Protein Kinase 3 biology Kinase Chemistry Activator (genetics) Molecular biology Rats Enzyme Activation Transcription Factor AP-1 Gene Expression Regulation Mitogen-activated protein kinase Tissue Plasminogen Activator biology.protein Female Signal transduction Follicle Stimulating Hormone Plasminogen activator hormones hormone substitutes and hormone antagonists |
| Zdroj: | Endocrine. 34(1-3) |
| ISSN: | 1355-008X |
| Popis: | We have analyzed a possible role of mitogen-activated protein kinase (MAPK) and activator protein-1 (AP-1) in the regulation of FSH-induced tissue type plasminogen activator (tPA) production in granulosa cells (GCs) prepared from DES-treated immature rats; Treatment of the cells in the presence of FSH with MAPK inhibitors, such as UO126 or SB203580, significantly decreased the FSH-induced tPA production, suggesting that multiple signaling pathways may be involved in FSH-regulated tPA expression. We further examined possible signaling action involved in FSH-activated ERK1/2 and p38 MAPK on tPA production, and observed that FSH receptor occupancy led to both ERK1/2 and p38 MAPK phosphorylation. Such action might be through a protein kinase A-dependent pathway because the observed activation was destroyed by the addition of its specific inhibitor H89 to the culture. The inhibition of ERK1/2 and p38 MAPK activation by their specific inhibitors remarkably reduced FSH-induced tPA mRNA and its protein production. We further examined whether AP-1 located in the tPA promoter is involved in FSH-regulated tPA production, and demonstrated that FSH significantly stimulated AP-1 expression, whereas inclusion of H89, UO126, or SB20358 in the culture significantly decreased FSH-induced AP-1 expression. In summary, FSH-induced ERK1/2 and p38 MAPK activation is capable of regulating tPA production in cultured primary GCs, and that the transcript factor AP-1 may be important in the regulation of FSH-induced tPA expression. |
| Databáze: | OpenAIRE |
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