| Autor: |
Sachindra S. T. Gamage, Thilanga N. Pahattuge, Harshani Wijerathne, Katie Childers, Swarnagowri Vaidyanathan, Uditha S. Athapattu, Lulu Zhang, Zheng Zhao, Mateusz L. Hupert, Rolf M. Muller, Judy Muller-Cohn, Janet Dickerson, Dylan Dufek, Brian V. Geisbrecht, Harsh Pathak, Ziyan Pessetto, Gregory N. Gan, Junseo Choi, Sunggook Park, Andrew K. Godwin, Malgorzata A. Witek, Steven A. Soper |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
Science advances. 8(39) |
| ISSN: |
2375-2548 |
| Popis: |
We report a microfluidic assay to select active severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) viral particles (VPs), which were defined as intact particles with an accessible angiotensin-converting enzyme 2 receptor binding domain (RBD) on the spike (S) protein, from clinical samples. Affinity selection of SARS-CoV-2 particles was carried out using injection molded microfluidic chips, which allow for high-scale production to accommodate large-scale screening. The microfluidic contained a surface-bound aptamer directed against the virus’s S protein RBD to affinity select SARS-CoV-2 VPs. Following selection (~94% recovery), the VPs were released from the chip’s surface using a blue light light-emitting diode (89% efficiency). Selected SARS-CoV-2 VP enumeration was carried out using reverse transcription quantitative polymerase chain reaction. The VP selection assay successfully identified healthy donors (clinical specificity = 100%) and 19 of 20 patients with coronavirus disease 2019 (COVID-19) (95% sensitivity). In 15 patients with COVID-19, the presence of active SARS-CoV-2 VPs was found. The chip can be reprogrammed for any VP or exosomes by simply changing the affinity agent. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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