A Standardized Collagen-Based Scaffold Improves Human Hepatocyte Shipment and Allows Metabolic Studies over 10 Days
| Autor: | Sabrina Ehnert, Andreas K. Nussler, Serene M. L. Lee, Liming Ge, Victor Häussling, Marc Ruoß, Leon Olde Damink, Frank Schügner |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Scaffold Bioengineering 02 engineering and technology drug-induced hepatotoxicity lcsh:Technology Article 03 medical and health sciences chemistry.chemical_compound medicine lcsh:QH301-705.5 Loss function lcsh:T Albumin cells shipment pre-clinical drug testing 021001 nanoscience & nanotechnology Cell biology Human hepatocyte 030104 developmental biology medicine.anatomical_structure natural collagen scaffolds lcsh:Biology (General) chemistry Hepatocyte Urea Animal studies 0210 nano-technology Function (biology) |
| Zdroj: | Bioengineering, Vol 5, Iss 4, p 86 (2018) Bioengineering Volume 5 Issue 4 |
| ISSN: | 2306-5354 |
| Popis: | Due to pronounced species differences, hepatotoxicity of new drugs often cannot be detected in animal studies. Alternatively, human hepatocytes could be used, but there are some limitations. The cells are not always available on demand or in sufficient amounts, so far there has been only limited success to allow the transport of freshly isolated hepatocytes without massive loss of function or their cultivation for a long time. Since it is well accepted that the cultivation of hepatocytes in 3D is related to an improved function, we here tested the Optimaix-3D Scaffold from Matricel for the transport and cultivation of hepatocytes. After characterization of the scaffold, we shipped cells on the scaffold and/or cultivated them over 10 days. With the evaluation of hepatocyte functions such as urea production, albumin synthesis, and CYP activity, we showed that the metabolic activity of the cells on the scaffold remained nearly constant over the culture time whereas a significant decrease in metabolic activity occurred in 2D cultures. In addition, we demonstrated that significantly fewer cells were lost during transport. In summary, the collagen-based scaffold allows the transport and cultivation of hepatocytes without loss of function over 10 days. |
| Databáze: | OpenAIRE |
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