Mutagenicity of ?-hydroxy N-nitrosamines in V79 Chinese hamster cells
| Autor: | M. Mochizuki, T. Anjo, E. Suzuki, Masashi Okada, M. Osabe |
|---|---|
| Rok vydání: | 1984 |
| Předmět: |
Alkylating Agents
endocrine system Cancer Research Nitrosamines Cell Survival Stereochemistry Hamster Mutagen Alkylation Hydroxylation medicine.disease_cause Chinese hamster Cell Line Structure-Activity Relationship chemistry.chemical_compound Cricetulus Phenols Cricetinae medicine Animals Hydroxymethyl Sulfhydryl Compounds Carcinogen biology Mutagenicity Tests fungi food and beverages General Medicine Hydrogen-Ion Concentration biology.organism_classification Oncology chemistry Nitrosamine Mutagens |
| Zdroj: | Journal of Cancer Research and Clinical Oncology. 108:290-295 |
| ISSN: | 1432-1335 0171-5216 |
| DOI: | 10.1007/bf00390460 |
| Popis: | Carcinogenic and mutagenic N-nitrosodialkylamines are metabolically activated through alpha-hydroxylation. The synthesis, chemical properties, and microbial mutagenicity of alpha-hydroxy N-nitrosamines have been reported previously. Potent mutagenicity of four N-nitroso-N-(hydroxymethyl)-alkylamines (alkyl = methyl, ethyl, propyl, and butyl) was demonstrated in the present study in V79 Chinese hamster cells, ouabain resistance being used as an indicator. All the compounds were strong mutagens in the absence of metabolic activation systems. The mutagenic and cytotoxic potencies correlated well with each other, and depended on the alkyl group, decreasing in potency in the following order: methyl greater than ethyl greater than propyl = butyl. Their alkylating reactivity was measured by alkylation of thiophenol, and a good linear relationship was observed between the mutagenic and cytotoxic potencies and their alkylating reactivity. The mutagenic and cytotoxic potencies of the alpha-hydroxy N-nitrosamines in V79 cells were well correlated with those of alpha-acetoxy and alpha-hydroperoxy N-nitrosamines with respect to the effect of alkyl group. The results obtained here supported further that alpha-hydroxy N-nitrosamine is the active species in the metabolic activation of N-nitrosodialkylamine. |
| Databáze: | OpenAIRE |
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