Application of creatinine-sensitive biosensor for hemodialysis control
| Autor: | S. V. Marchenko, O. A. Zinchenko, A.S. Pavlyuchenko, A. L. Kukla, Anna V. El'skaya, E.K. Krasyuk, T.A. Sergeyeva, A. P. Soldatkin |
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| Rok vydání: | 2012 |
| Předmět: |
Creatinine deiminase
Potentiometric biosensor medicine.medical_treatment Biomedical Engineering Biophysics Biosensing Techniques macromolecular substances Sensitivity and Specificity chemistry.chemical_compound Renal Dialysis Electrochemistry medicine Humans In patient Dialysis Creatinine Chromatography Chemistry technology industry and agriculture General Medicine Body Fluids Highly sensitive Biochemistry Kidney Failure Chronic Hemodialysis Biosensor Biotechnology |
| Zdroj: | Biosensors and Bioelectronics. 35:466-469 |
| ISSN: | 0956-5663 |
| Popis: | The highly sensitive and selective potentiometric biosensor for creatinine determination has been developed by us earlier. In it, pH-sensitive field effect transistors were used as transducer and immobilized creatinine deiminase (EC 3.5.4.21)—as a biosensitive element. In the work presented, we optimized this biosensor for creatinine analysis in real samples of dialysate in patients with renal failure. The optimized version of biosensor was applied for on-line monitoring of the level of creatinine in the patient's dialysate fluid in the course of dialysis session. High correlation between the biosensor analysis and traditional Jaffe method was demonstrated. |
| Databáze: | OpenAIRE |
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