Molecular typing and genetic relatedness of 72 clinical Candida albicans isolates from poultry
| Autor: | Liu Yanwei, Heping Zhang, Jinkun Yan, Chengrui Zhao, Liu Na, Huanzhang Liu, Liu Jianchai |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
China Genotype 030106 microbiology Population Biology Microbiology Poultry 03 medical and health sciences Candida albicans Animals Humans Internal transcribed spacer education Genotyping Phylogeny Genetics education.field_of_study General Veterinary Molecular epidemiology Phylogenetic tree Candidiasis General Medicine biology.organism_classification Corpus albicans Molecular Typing Multilocus sequence typing Chickens Multilocus Sequence Typing |
| Zdroj: | Veterinary microbiology. 214 |
| ISSN: | 1873-2542 |
| Popis: | Candida albicans is the most prevalent opportunistic fungus of humans and animals. While most studies focus on human isolates, they rarely focus on poultry isolates. In this study, C. albicans strains were recovered from poultry in the southern Hebei Province (China) and identified. Molecular typing and analyses were performed to understand the molecular epidemiology and genetic relatedness of the strains. The fungi were isolated from live birds with presumed candidiasis or their corpses. The isolates were identified based on morphology, differential medium culture, and rDNA internal transcribed spacer sequencing. The identified C. albicans strains were analyzed by ABC genotyping and multilocus sequence typing. Clonal groups were identified using the eBURST (version 3.0) software, and an UPGMA phylogenetic tree was constructed using the MEGA (version 6.06) software. Overall, 72 isolates were divided into three genotypes (A, B, and C), 48 novel sequence types (STs), five groups with 10 singletons, and four clades. Results indicated that candidiasis is common in poultry in the southern Hebei Province, and that the genetic composition of the C. albicans poultry population from the area is relatively complicated. Based on the eBURST analysis for the STs in this study and others, we suggest that C. albicans poultry isolates were relatively independent but not completely separated from human isolates. The strains with the same or closely related genotypes but recovered from both birds and humans could have transferred and evolved between the two types of host. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect