Expression profiling of the solute carrier gene family in chicken intestine from the late embryonic to early post-hatch stages
| Autor: | O. Crasta, Yan Zhang, Huifeng Li, Kenneth E. Webb, Derek A. Emmerson, Eric A. Wong, Elizabeth R. Gilbert |
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| Rok vydání: | 2008 |
| Předmět: |
Male
Amino Acid Transport Systems Monosaccharide Transport Proteins Down-Regulation Chick Embryo Biology Intestine Small Genetics medicine Animals Gene family Gene Oligonucleotide Array Sequence Analysis Regulation of gene expression chemistry.chemical_classification Reverse Transcriptase Polymerase Chain Reaction Gene Expression Profiling Gene Expression Regulation Developmental General Medicine Molecular biology Small intestine Up-Regulation Solute carrier family Amino acid Gene expression profiling medicine.anatomical_structure Biochemistry chemistry Multigene Family biology.protein RNA Animal Nutritional Physiological Phenomena Animal Science and Zoology Chickens GLUT5 |
| Zdroj: | Animal Genetics. 39:407-424 |
| ISSN: | 1365-2052 0268-9146 |
| DOI: | 10.1111/j.1365-2052.2008.01744.x |
| Popis: | Intestinal development during late embryogenesis and early post-hatch has a long-term influence on digestive and absorptive capacity in chickens. The objective of this research was to obtain a global view of intestinal solute carrier (SLC) gene family member expression from late embryogenesis until 2 weeks post-hatch with a focus on SLC genes involved in uptake of sugars and amino acids. Small intestine samples from male chicks were collected on embryonic days 18 (E18) and 20 (E20), day of hatch and days 1, 3, 7 and 14 post-hatch. The expression profiles of 162 SLC genes belonging to 41 SLC families were determined using Affymetrix chicken genome microarrays. The majority of SLC genes showed little or no difference in level of expression during E18-D14. A number of well-known intestinal transporters were upregulated between E18 and D14 including the amino acid transporters rBAT, y(+)LAT-2 and EAAT3, the peptide transporter PepT1 and the sugar transporters SGLT1, GLUT2 and GLUT5. The amino acid transporters CAT-1 and CAT-2 were downregulated. In addition, several glucose and amino acid transporters that are novel to our understanding of nutrient absorption in the chicken intestine were discovered through the arrays (SGLT6, SNAT1, SNAT2 and AST). These results represent a comprehensive characterization of the expression profiles of the SLC family of genes at different stages of development in the chicken intestine and lay the ground work for future nutritional studies. |
| Databáze: | OpenAIRE |
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