MicroRNA-181 inhibits proliferation and promotes apoptosis of chondrocytes in osteoarthritis by targeting PTEN
| Autor: | Jian Zou, Zi-Hui Zhou, Xiao-Feng Wu |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine Poly ADP ribose polymerase Blotting Western Apoptosis Biology Real-Time Polymerase Chain Reaction Biochemistry Flow cytometry 03 medical and health sciences Chondrocytes 0302 clinical medicine Osteoarthritis microRNA medicine Humans PTEN MTT assay RNA Messenger RNA Small Interfering Molecular Biology Cells Cultured Cell Proliferation medicine.diagnostic_test Reverse Transcriptase Polymerase Chain Reaction Cell growth Cell Cycle PTEN Phosphohydrolase Cell Biology Middle Aged Flow Cytometry Prognosis Molecular biology Blot MicroRNAs 030104 developmental biology Case-Control Studies 030220 oncology & carcinogenesis Cancer research biology.protein Female Signal Transduction |
| Zdroj: | Biochemistry and Cell Biology. 95:437-444 |
| ISSN: | 1208-6002 0829-8211 |
| DOI: | 10.1139/bcb-2016-0078 |
| Popis: | Objective: To investigate the effects of microRNA-181 (miR-181) on the proliferation and apoptosis of chondrocytes in osteoarthritis (OA) by targeting PTEN. Methods: The chondrocytes in logarithmic growth phase were selected and divided into 6 test groups: the normal, blank, negative control, miR-181 mimic, miR-181 inhibitor, and miR-181 inhibitor + PTEN-siRNA groups. Reverse transcription qPCR was used to detect the expressions of miR-181 and PTEN mRNA. MTT assay and flow cytometry were performed to detect cell proliferation and apoptosis. The protein expressions of PARP and caspase-3 and the activity of MMP-2 and MMP-9 were detected by Western blotting and gelatin zymography assay. Results: The miR-181 mimic group showed increased miR-181 expression and decreased PTEN expression compared with the other 5 groups. Also, by comparison with the other 5 groups, the cell proliferation rate declined and the rate of cell apoptosis was elevated in the miR-181 mimic group. The MiR-181 mimic group showed remarkably increased protein expression of caspase-3 and PARP compared with the other 5 groups. The activity of MMP-2 and MMP-9 was higher in the miR-181 mimic group than the other 5 groups. Conclusion: MiR-181 could up-regulate the expressions of caspase-3, PARP, MMP-2, and MMP-9, and thereby inhibit cell proliferation and promote apoptosis of chondrocytes in OA by targeting PTEN. |
| Databáze: | OpenAIRE |
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