Improvement of Peroxyl Radical Scavenging Bioassay Based on Bactericidal Action oftert-Butyl Peroxyl Radical
| Autor: | Shigenori Ueno, Masami Sato, Yoshihiro Tsuriya, Toshiyuki Kawasumi |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Antioxidant
biology Chemistry Dimethyl sulfoxide medicine.medical_treatment Organic Chemistry Biological activity General Medicine biology.organism_classification Ascorbic acid Applied Microbiology and Biotechnology Biochemistry Medicinal chemistry Methemoglobin Analytical Chemistry chemistry.chemical_compound medicine Organic chemistry Bioassay Molecular Biology Scavenging Biotechnology Enterococcus faecium |
| Zdroj: | Bioscience, Biotechnology, and Biochemistry. 63:577-580 |
| ISSN: | 1347-6947 0916-8451 |
| DOI: | 10.1271/bbb.63.577 |
| Popis: | An improved bioassay for measurement of peroxyl radical scavenging activity(1,2)) is described. The modifications included the use of (1) Enterococcus faecium strain S, (2) 25% dimethyl sulfoxide, (3) L-ascorbic acid for the termination of the bactericidal reaction, and (4) the concentration of antioxidants that gives 75% of the log (colony forming unit/ml) of the control reaction (without methemoglobin or tert-butyl hydroperoxide), for the expression of antioxidant activity. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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