Alpha-crystallin promotes rat retinal neurite growth on myelin substrates in vitro
| Autor: | Nan Wu, Yan Hua Wang, Zheng Qin Yin, Dong Wu Wang, Yi Wang |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Neurite
Cell Survival Neurogenesis Nogo Proteins Blotting Western Cell Count Inhibitory postsynaptic potential Cellular and Molecular Neuroscience chemistry.chemical_compound Myelin medicine Neurites Animals Microscopy Phase-Contrast Rats Long-Evans alpha-Crystallins Axon Bovine serum albumin Cells Cultured Myelin Sheath Cell Proliferation biology Regeneration (biology) Retinal General Medicine Anatomy eye diseases Sensory Systems In vitro Cell biology Rats Ophthalmology Myelin-Associated Glycoprotein medicine.anatomical_structure nervous system chemistry Animals Newborn biology.protein sense organs Myelin Proteins Retinal Neurons |
| Zdroj: | Ophthalmic research. 45(3) |
| ISSN: | 1423-0259 |
| Popis: | Background: Myelin-associated molecules are major impediments to axon regeneration after optic nerve injury. Intravitreal injection of α-crystallin can protect axons from optic nerve degeneration after crushing in rats. Objectives: Our purpose was to investigate whether α-crystallin could counteract the inhibitory effect of myelin and promote neurite growth. Methods: Newborn rat retinal neurons were cultured on myelin-coated dishes with DMEM containing α-crystallin (10–4 g/l) or bovine serum albumin. The density of neurons with neurites and the length of the longest neurite of the cells were analyzed on days 1, 3 and 5. Results: Cultures containing α-crystallin had significantly higher neurite-containing cell densities, and the neurites were significantly longer compared with cultures containing bovine serum albumin. These findings indicated that α-crystallin could counteract the effect of myelin inhibitory factors and stimulate neurite growth. |
| Databáze: | OpenAIRE |
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