Tandem Mass Spectrometry Imaging and in Situ Characterization of Bioactive Wood Metabolites in Amazonian Tree Species Sextonia rubra
| Autor: | Christophe Duplais, Gregory L. Fisher, Nadine Amusant, Serge Della-Negra, Alain Brunelle, Emeline Houël, Tingting Fu, David Touboul |
|---|---|
| Přispěvatelé: | Université Paris-Sud - Paris 11 (UP11), Institut de Physique Nucléaire d'Orsay (IPNO), Centre National de la Recherche Scientifique (CNRS)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Université Paris-Sud - Paris 11 (UP11), Université Paris-Sud - Paris 11 (UP11)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS), Institut de Chimie des Substances Naturelles (ICSN), Centre National de la Recherche Scientifique (CNRS), UMR8608, Ecologie des forêts de Guyane (UMR ECOFOG), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-AgroParisTech-Université de Guyane (UG)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA), Ecologie des forêts de Guyane (ECOFOG), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Université des Antilles et de la Guyane (UAG)-AgroParisTech-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
In situ
K50 - Technologie des produits forestiers Surface Properties Metabolite F60 - Physiologie et biochimie végétale Bois de coeur Métabolite 02 engineering and technology Spectrométrie de masse Alkenes Mass spectrometry Tandem mass spectrometry 01 natural sciences Analytical Chemistry Trees chemistry.chemical_compound Bois Lauraceae Acetals Tandem Mass Spectrometry [CHIM.ANAL]Chemical Sciences/Analytical chemistry Forêt tropicale humide ComputingMilieux_MISCELLANEOUS [PHYS]Physics [physics] Biological Products Natural product Chromatography Tandem Molecular Structure 010401 analytical chemistry 021001 nanoscience & nanotechnology Wood 0104 chemical sciences Characterization (materials science) Secondary ion mass spectrometry chemistry Alkynes U30 - Méthodes de recherche 0210 nano-technology Chromatography Liquid |
| Zdroj: | Anal.Chem. Anal.Chem., 2018, 90 (12), pp.7535-7543. ⟨10.1021/acs.analchem.8b01157⟩ Analytical Chemistry Analytical Chemistry, American Chemical Society, 2018, 90 (12), pp.7535-7543. ⟨10.1021/acs.analchem.8b01157⟩ |
| ISSN: | 0003-2700 1520-6882 |
| DOI: | 10.1021/acs.analchem.8b01157⟩ |
| Popis: | International audience; Driven by a necessity for confident molecular identification at high spatial resolution, a new time-of-flight secondary ion mass spectrometry (TOF-SIMS) tandem mass spectrometry (tandem MS) imaging instrument has been recently developed. In this paper, the superior MS/MS spectrometry and imaging capability of this new tool is shown for natural product study. For the first time, via in situ analysis of the bioactive metabolites rubrenolide and rubrenolide in Amazonian tree species Sextonia rubra (Lauraceae), we were able both to analyze and to image by tandem MS the molecular products of natural biosynthesis. Despite the low abundance of the metabolites in the wood sample(s), efficient MS/MS analysis of these gamma-lactone compounds was achieved, providing high confidence in the identification and localization. In addition, tandem MS imaging minimized the mass interferences and revealed specific localization of these metabolites primarily in the ray parenchyma cells but also in certain oil cells and, further, revealed the presence of previously unidentified gamma-lactone, paving the way for future studies in biosynthesis. |
| Databáze: | OpenAIRE |
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