Molecular response of Deinococcus radiodurans to simulated microgravity explored by proteometabolomic approach
| Autor: | Yuko Kawaguchi, Ruth Hemmersbach, Wolfram Weckwerth, Felix M. Fuchs, Ralf Moeller, Akihiko Yamagishi, Tetyana Milojevic, Emanuel Ott |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Proteomics Signal processing Reduced Gravity 030106 microbiology lcsh:Medicine Molecular Response Mass Spectrometry Article Fight-or-flight response 03 medical and health sciences Strahlenbiologie Extremophiles Bacterial Proteins Deinococcus radiodurans Stress Physiological Extracellular Metabolomics lcsh:Science simulated microgravity Weightlessness Simulation Bacterial systems biology Multidisciplinary biology Chemistry lcsh:R Gene Expression Regulation Bacterial Space Flight biology.organism_classification Adaptation Physiological 030104 developmental biology Simulated microgravity Biophysics Gravitationsbiologie lcsh:Q Deinococcus Cell envelope Clinostat |
| Zdroj: | Scientific Reports Scientific Reports, Vol 9, Iss 1, Pp 1-13 (2019) |
| ISSN: | 2045-2322 |
| Popis: | Regarding future space exploration missions and long-term exposure experiments, a detailed investigation of all factors present in the outer space environment and their effects on organisms of all life kingdoms is advantageous. Influenced by the multiple factors of outer space, the extremophilic bacterium Deinococcus radiodurans has been long-termly exposed outside the International Space Station in frames of the Tanpopo orbital mission. The study presented here aims to elucidate molecular key components in D. radiodurans, which are responsible for recognition and adaptation to simulated microgravity. D. radiodurans cultures were grown for two days on plates in a fast-rotating 2-D clinostat to minimize sedimentation, thus simulating reduced gravity conditions. Subsequently, metabolites and proteins were extracted and measured with mass spectrometry-based techniques. Our results emphasize the importance of certain signal transducer proteins, which showed higher abundances in cells grown under reduced gravity. These proteins activate a cellular signal cascade, which leads to differences in gene expressions. Proteins involved in stress response, repair mechanisms and proteins connected to the extracellular milieu and the cell envelope showed an increased abundance under simulated microgravity. Focusing on the expression of these proteins might present a strategy of cells to adapt to microgravity conditions. |
| Databáze: | OpenAIRE |
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