Mesenchymal stem cells increase antioxidant capacity in intestinal ischemia/reperfusion damage

Autor: E. Karaöz, Enis Uluçam, Mustafa Inan, Filiz Sanal, Elvan Bakar, Aysegul Cerkezkayabekir, C. Subaşı
Přispěvatelé: İstinye Üniversitesi, Hastane, Subasi Demir, Cansu, Karaoz, Erdal
Rok vydání: 2016
Předmět:
0301 basic medicine
Male
Pharmacology
medicine.disease_cause
Antioxidants
Local And Systemic Administration
chemistry.chemical_compound
0302 clinical medicine
Ischemia
Malondialdehyde
Intestine
Small
Superior mesenteric artery
chemistry.chemical_classification
biology
Glutathione peroxidase
Antioxidant Capacity
General Medicine
Intestines
Mesenchymal Stem Cell
medicine.anatomical_structure
030220 oncology & carcinogenesis
Reperfusion Injury
Small Intestine
Cytokines
Superoxide dismutase
03 medical and health sciences
Mesenteric Artery
Superior
medicine.artery
medicine
Animals
business.industry
Superoxide Dismutase
Tumor Necrosis Factor-alpha
Mesenchymal stem cell
Mesenchymal Stem Cells
medicine.disease
Small intestine
Rats
Oxidative Stress
030104 developmental biology
chemistry
Reperfusion
Pediatrics
Perinatology and Child Health
Immunology
biology.protein
Surgery
business
Oxidative stress
Zdroj: Journal of pediatric surgery. 52(7)
ISSN: 1531-5037
Popis: Background: Mesenchymal stemcells (MSCs) may have beneficial effects in reversing intestinal damage resulting fromcirculatory disorders. The hypothesis of this study is thatMSCs increase antioxidant capacity of small bowel tissue following intestinal ischemia reperfusion (I/R) damage. Methods: A total of 100 rats were used for the control group and three experimental groups, as follows: the sham control, local MSC, and systemic MSC groups. Each group consisted of 10 animals on days 1, 4, and 7 of the experiment. Ischemiawas established by clamping the superior mesenteric artery (SMA) for 45min; following this, reperfusion was carried out for 1, 4, and 7 days in all groups. In the local and systemic groups, MSCs were administered intravenously and locally just after the ischemia, and they were investigated after 1, 4, and 7 days. The superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (Gpx) activities, as well as malondialdehyde (MDA) and total protein levels, were measured. Histopathological analysis was performed using light and electron microscopy. The indicators of proliferation from the effects of anti-and pro-inflammatory cytokines were evaluated using immunohistochemistry. Results: MDA was increased (P < 0.05) in the sham control group and decreased (P < 0.05) in the MSC groups. SOD, CAT, and Gpx were decreased in the localMSC group (P < 0.05). The highest level of amelioration was observed on day 7 in the local MSC group via light and electron microscopy. It was found that the MSCs arrived at the damaged intestinal wall in the MSC groups immediately after injection. Pro-inflammatory cytokines interleukin-1 beta(IL1 beta), transforming growth factor-beta 1 (TGF beta 1), tumor necrosis factor-alpha (TNF alpha), IL6, MIP2, and MPO decreased (P < 0.05), while anti-inflammatory cytokines EP3 and IL1ra increased (p < 0.05) in the local and systemic MSC groups. In addition, proliferation indicators, such as PCNA and KI67, increased (P < 0.05) in the local and systemic MSC groups. Conclusions: Parallel to our hypothesis, MSC increases the antioxidant capacity of small bowel tissue after intestinal I/R damage. The MSCs migrated to the reperfused small intestine by homing and reduced oxidative stress via the effects of SOD, CAT, and Gpx, as well as reducing the MDA level; thus, they could increase antioxidant capacity of intestine and have a therapeutic effect on the damaged tissue. We think that this effectwas achieved via scavenging of oxygen radicals, suppression of pro-inflammatory cytokines, and increasing the expression of anti-inflammatory cytokines. (C) 2017 Elsevier Inc. All rights reserved. Scientific and Technological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [112S124] The authorswould like to thank the Scientific and Technological Research Council of Turkey (TUBITAK), as this project was supported by grants from this institution (no. 112S124). The authors would also like to thank the staff of KOGEM, Kocaeli, Turkey, for their support in the isolation, characterization, identification, and monitoring of MSCs, as well as the preparation of histological sections; the Electron Microscope Laboratory of Osmangazi University, Eskisehir, Turkey, for preparation of electron microscopic sections; and the Experimental Animal Breeding and Research Unit of Trakya University, Edirne, Turkey for the care and preparation of rats for the experimental surgical procedure. WOS:000405362500025 28118930 Q2
Databáze: OpenAIRE
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