Identification and Characterization of Differentially-Regulated Type IVb Pilin Genes Necessary for Predation in Obligate Bacterial Predators

Autor:	Edouard Jurkevitch, Shmuel Pietrokovski, Margarita Petrenko, Ofir Avidan, René Becker, Michael W. Linscheid, Sebastian Beck
Jazyk:	angličtina
Rok vydání:	2017
Předmět:	0301 basic medicine Operon Science 030106 microbiology Bacterial Adhesion Article Bacterial genetics Microbiology 03 medical and health sciences Bacterial Proteins Sigma factor Gene Genetics Genes Essential Multidisciplinary biology Obligate Computational Biology Gene Expression Regulation Bacterial Genomics Bdellovibrio bacteriovorus biology.organism_classification Bdellovibrio Fimbriae Bacterial Pilin biology.protein Medicine Fimbriae Proteins
Zdroj:	Scientific Reports, Vol 7, Iss 1, Pp 1-12 (2017) Scientific Reports
ISSN:	2045-2322
Popis:	Bdellovibrio bacteriovorus is an obligate predator of bacteria that grows and divides within the periplasm of its prey. Functions involved in the early steps of predation have been identified and characterized, but mediators of prey invasion are still poorly detailed. By combining omics data available for Bdellovibrio and like organisms (BALO’s), we identified 43 genes expressed in B. bacteriovorus during the early interaction with prey. These included genes in a tight adherence (TAD) operon encoding for two type IVb fimbriae-like pilin proteins (flp1 and flp2), and their processing and export machinery. Two additional flp genes (flp3 and flp4) were computationally identified at other locations along the chromosome, defining the largest and most diverse type IVb complement known in bacteria to date. Only flp1, flp2 and flp4 were expressed; their respective gene knock-outs resulted in a complete loss of the predatory ability without losing the ability to adhere to prey cells. Additionally, we further demonstrate differential regulation of the flp genes as the TAD operon of BALOs with different predatory strategies is controlled by a flagellar sigma factor FliA, while flp4 is not. Finally, we show that FliA, a known flagellar transcriptional regulator in other bacteria, is an essential Bdellovibrio gene.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1fb33a58cb76d32011c3ba7ac65a8ce8 https://doaj.org/article/8307e4567f9247688759ac79d1776d91 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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