miR-92a is upregulated in cervical cancer and promotes cell proliferation and invasion by targeting FBXW7
| Autor: | Lei Mao, Liangfang Shen, Yang Li, Huizhi Yu, Bing Wang, Chuanyi Zhou |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
F-Box-WD Repeat-Containing Protein 7
Ubiquitin-Protein Ligases Biophysics Uterine Cervical Neoplasms Cell Cycle Proteins Biology medicine.disease_cause Biochemistry Downregulation and upregulation Cell Line Tumor microRNA medicine Gene silencing Humans Molecular Biology 3' Untranslated Regions Cell Proliferation Cervical cancer Gene knockdown Cell growth F-Box Proteins Cell Cycle Cell Biology Cell cycle medicine.disease Cell biology Up-Regulation Gene Expression Regulation Neoplastic MicroRNAs Female Carcinogenesis HeLa Cells |
| Zdroj: | Biochemical and biophysical research communications. 458(1) |
| ISSN: | 1090-2104 |
| Popis: | MicroRNAs (miRNAs) are involved in the cervical carcinogenesis and progression. In this study, we investigated the role of miR-92a in progression and invasion of cervical cancer. MiR-92a was significantly upregulated in cervical cancer tissues and cell lines. Overexpression of miR-92a led to remarkably enhanced proliferation by promoting cell cycle transition from G1 to S phase and significantly enhanced invasion of cervical cancer cells, while its knockdown significantly reversed these cellular events. Bioinformatics analysis suggested F-box and WD repeat domain-containing 7 (FBXW7) as a novel target of miR-92a, and miR-92a suppressed the expression level of FBXW7 mRNA by direct binding to its 3'-untranslated region (3'UTR). Expression of miR-92a was negatively correlated with FBXW7 in cervical cancer tissues. Furthermore, Silencing of FBXW7 counteracted the effects of miR-92a suppression, while its overexpression reversed oncogenic effects of miR-92a. Together, these findings indicate that miR-92a acts as an onco-miRNA and may contribute to the progression and invasion of cervical cancer, suggesting miR-92a as a potential novel diagnostic and therapeutic target of cervical cancer. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect