Performance of Four Transport and Storage Systems for Molecular Detection of Multidrug-Resistant Tuberculosis

Autor: Anandi Martin, Rina Andrianiavomikotroka, Lucilaine Ferrazoli, Peter Vandamme, Sarman Singh, Belén Rocío Imperiale, Marie Sylvianne Rabodoarivelo, Voahangy Rasolofo, Angela Pires Brandão, Juan Carlos Palomino, Nora Morcillo, Parveen Kumar
Jazyk: angličtina
Rok vydání: 2015
Předmět:
MTBDRPLUS ASSAY
Genotyping Techniques
Antitubercular Agents
Ciencias de la Salud
lcsh:Medicine
Transportation
Drug resistance
Polymerase Chain Reaction
Ribotyping
Drug Resistance
Multiple
Bacterial
Tuberculosis
Multidrug-Resistant
Medicine and Health Sciences
Molecular diagnostic techniques
TRANSPORT AND STORAGE SYSTEM
lcsh:Science
Multidisciplinary
biology
DRUG RESISTANT M. TUBERCULOSIS
Bacterial Typing Techniques
Molecular Diagnostic Techniques
purl.org/becyt/ford/3 [https]
MYCOBACTERIUM-TUBERCULOSIS
Brazil
Research Article
DNA
Bacterial
Paper
CIENCIAS MÉDICAS Y DE LA SALUD
TISSUES
Tuberculosis
Genotype
Preservation
Biological
Argentina
India
Computational biology
Microbial Sensitivity Tests
Reference laboratory
MOLECULAR DRUG RESISTANCE DETECTION
Sensitivity and Specificity
Microbiology
Specimen Handling
Mycobacterium tuberculosis
purl.org/becyt/ford/3.3 [https]
Postal service
medicine
Humans
Postal Service
Retrospective Studies
RIFAMPIN
GenoType MTBDRplus
Bacteriological Techniques
IDENTIFICATION
Ethanol
STRAINS
lcsh:R
CARDS
Sputum
Extensively drug-resistant tuberculosis
DNA
biology.organism_classification
medicine.disease
SPECIMENS
Enfermedades Infecciosas
Multiple drug resistance
lcsh:Q
Glass
Filtration
Zdroj: PLoS ONE, Vol 10, Iss 10, p e0139382 (2015)
PLoS ONE
CONICET Digital (CONICET)
Consejo Nacional de Investigaciones Científicas y Técnicas
instacron:CONICET
PLOS ONE
ISSN: 1932-6203
Popis: Background Detection of drug-resistant tuberculosis is essential for the control of the disease but it is often hampered by the limitation of transport and storage of samples from remote locations to the reference laboratory. We performed a retrospective field study to evaluate the performance of four supports enabling the transport and storage of samples to be used for molecular detection of drug resistance using the GenoType MTBDRplus. Methods Two hundred Mycobacterium tuberculosis strains were selected and spotted on slides, FTA cards, GenoCards, and in ethanol. GenoType MTBDRplus was subsequently performed with the DNA extracted from these supports. Sensitivity and specificity were calculated and compared to the results obtained by drug susceptibility testing. Results For all supports, the overall sensitivity and specificity for detection of resistance to RIF was between 95% and 100%, and for INH between 95% and 98%. Conclusion The four transport and storage supports showed a good sensitivity and specificity for the detection of resistance to RIF and INH in M. tuberculosis strains using the GenoType MTBDRplus. These supports can be maintained at room temperature and could represent an important alternative cost-effective method useful for rapid molecular detection of drugresistant TB in low-resource settings. Fil: Rabodoarivelo, Marie Sylvianne. University of Ghent; Bélgica Fil: Imperiale, Belén Rocío. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Provincia de Buenos Aires. Ministerio de Salud. Hospital "Dr. Antonio A. Cetrángolo"; Argentina Fil: Andrianiavomikotroka, Rina. Institut Pasteur de Madagascar; Madagascar Fil: Brandao, Angela. Fundación Oswaldo Cruz; Brasil Fil: Kumar, Parveen. All India Institute of Medical Sciences; India Fil: Singh, Sarman. All India Institute of Medical Sciences; India Fil: Ferrazoli, Lucilaine. Instituto Adolfo Lutz Núcleo de Tuberculose e Micobacteriosis Sao Paulo; Brasil Fil: Morcillo, Nora. Provincia de Buenos Aires. Ministerio de Salud. Hospital "Dr. Antonio A. Cetrángolo"; Argentina Fil: Rasolofo, Voahangy. Institut Pasteur de Madagascar; Madagascar Fil: Palomino, Juan Carlos. University of Ghent; Bélgica Fil: Vandamme, Peter. University of Ghent; Bélgica Fil: Martin, Anandi. University of Ghent; Bélgica
Databáze: OpenAIRE
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