A chicken protein hydrolysate exerts anti-atherosclerotic effect beyond plasma cholesterol-lowering activity in Apoe−/− mice
| Autor: | Rolf K. Berge, Rasa Slizyte, Pavol Bohov, Thomas A. Aloysius, Anders Lund, Ana Karina Carvajal, Bodil Bjørndal |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty chicken protein hydrolysate Apolipoprotein B Inflammation 030204 cardiovascular system & hematology Chicken protein hydrolysate Hydrolysate 03 medical and health sciences 0302 clinical medicine Plasma cholesterol medicine.artery Internal medicine Enzymatic hydrolysis Casein medicine fatty acid composition TX341-641 Original Research chemistry.chemical_classification Aorta biology Chemistry Nutrition. Foods and food supply atherosclerosis inflammation cytokines Fatty acid Atherosclerosis 030104 developmental biology Endocrinology biology.protein Cytokines medicine.symptom Fatty acid composition Food Science |
| Zdroj: | Food Science & Nutrition Food Science & Nutrition, Vol 8, Iss 7, Pp 3052-3060 (2020) Food Science & Nutrition, 8(7):3052-3060 |
| Popis: | Chicken protein hydrolysates (CPHs) generated from rest raw materials through enzymatic hydrolysis using Corolase PP or Alcalase were shown to reduce inflammation and stimulate hepatic mitochondrial fatty acid oxidation in high‐fat‐fed mice. This study investigates the effect of CPH diets in atherosclerosis‐prone apolipoprotein E‐deficient (Apoe−/−) mice. Apoe−/− mice were divided into three groups of 12 animals and fed high‐fat diets with casein (control), Alcalase CPH, or Corolase PP CPH. After 12 weeks, mice were sacrificed, blood samples were collected, and aorta was dissected for subsequent én face analysis. Mice fed Corolase PP CPH but not Alcalase CPH had significantly lower % atherosclerotic plaque area in the aortic arch compared to controls (p = .015 and p = .077, respectively). Plasma and liver cholesterol and triacylglycerol remained constant, but levels of the fatty acid C20:5n‐3 were increased, accompanied by an elevated delta‐5 desaturase index in both CPHs groups. Moreover, a significant reduction of plasma MCP‐1 was detected in Corolase PP CPH compared to control. Overall, our data show that protein hydrolysates from chicken reduced atherosclerosis and attenuated systemic risk factors related to atherosclerotic disorders, not related to changes in the level of plasma cholesterol. Chicken protein hydrolysates (CPHs) generated from rest raw materials through enzymatic hydrolysis using Corolase PP or Alcalase were shown to reduce aortic atherosclerotic plaque formation compared to a casein control in Apoe‐/‐ mice fed a high‐fat diet for 12 weeks. This reduction was independent of plasma lipid levels, but may be linked to effects on fatty acid composition and systemic inflammatory processes. |
| Databáze: | OpenAIRE |
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