JAG1-Mediated Notch Signaling Regulates Secretory Cell Differentiation of the Human Airway Epithelium
| Autor: | Jonna Heldrich, Vanessa Arbelaez, Matthew S. Walters, Ronald G. Crystal, Kazunori Gomi, Allison M. Rogalski, Robert J. Kaner, Michelle R. Staudt, Jacqueline Salit |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Cancer Research JAG1 Cellular differentiation Blotting Western Notch signaling pathway Fluorescent Antibody Technique Respiratory Mucosa Biology Epithelium Article 03 medical and health sciences medicine Humans Progenitor cell Receptors Notch Reverse Transcriptase Polymerase Chain Reaction Sequence Analysis RNA Gene Expression Profiling Stem Cells Cell Differentiation Epithelial Cells Cell Biology Cell biology 030104 developmental biology medicine.anatomical_structure Notch proteins Jagged-1 Protein RNA Interference Stem cell Signal Transduction |
| Zdroj: | Stem cell reviews and reports. 12(4) |
| ISSN: | 2629-3277 |
| Popis: | Basal cells (BC) are the stem/progenitor cells of the human airway epithelium capable of differentiating into secretory and ciliated cells. Notch signaling activation increases BC differentiation into secretory cells, but the role of individual Notch ligands in regulating this process in the human airway epithelium is largely unknown. The objective of this study was to define the role of the Notch ligand JAG1 in regulating human BC differentiation. JAG1 over-expression in BC increased secretory cell differentiation, with no effect on ciliated cell differentiation. Conversely, knockdown of JAG1 decreased expression of secretory cell genes. These data demonstrate JAG1-mediated Notch signaling regulates differentiation of BC into secretory cells. |
| Databáze: | OpenAIRE |
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