Rapid Diagnosis of Babesia gibsoni by Point-of-Need Testing by Insulated Isothermal PCR in Dogs at High Risk of Infection

Autor: Kirsten L. Cooke, P.C. Crawford, Sylvia J Tucker, Julie Levy, P Frenzer, S.K. Kirk
Rok vydání: 2018
Předmět:
Zdroj: Journal of Veterinary Internal Medicine
ISSN: 0891-6640
DOI: 10.1111/jvim.15033
Popis: Background Dogs seized by law enforcement agencies during dogfighting investigations are at increased risk of Babesia gibsoni infection. A rapid and cost‐effective diagnostic test would increase the feasibility of mass screening of dogs for infection and monitoring treatment efficacy in B. gibsoni‐infected dogs. Objective To determine the performance of a point‐of‐need insulated isothermal PCR (iiPCR) test for diagnosis of B. gibsoni in dogs rescued in dogfighting investigations. Animals Two hundred and thirty‐three dogs seized in dogfighting investigations. Methods Cross‐sectional study. Whole blood samples were tested for B. gibsoni and Babesia spp. by iiPCR. Results were compared to a reference standard comprised of concordant results from real‐time PCR in a commercial diagnostic laboratory and antibody titers. Results The iiPCR system was quick to learn, portable, and had a short processing time of
Databáze: OpenAIRE
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