Rapid Diagnosis of Babesia gibsoni by Point-of-Need Testing by Insulated Isothermal PCR in Dogs at High Risk of Infection
Autor: | Kirsten L. Cooke, P.C. Crawford, Sylvia J Tucker, Julie Levy, P Frenzer, S.K. Kirk |
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Rok vydání: | 2018 |
Předmět: |
0301 basic medicine
Pit bull medicine.medical_specialty 040301 veterinary sciences Point-of-Care Systems Babesia Infectious Disease Standard Article Real-Time Polymerase Chain Reaction Canine iiPCR 0403 veterinary science 03 medical and health sciences Dogs Babesiosis Internal medicine medicine Animals Dog Diseases Diagnostic laboratory Mass screening General Veterinary biology Babesia gibsoni business.industry Risk of infection Antibody titer Reproducibility of Results 04 agricultural and veterinary sciences medicine.disease biology.organism_classification Standard Articles Confidence interval Cross-Sectional Studies 030104 developmental biology SMALL ANIMAL business |
Zdroj: | Journal of Veterinary Internal Medicine |
ISSN: | 0891-6640 |
DOI: | 10.1111/jvim.15033 |
Popis: | Background Dogs seized by law enforcement agencies during dogfighting investigations are at increased risk of Babesia gibsoni infection. A rapid and cost‐effective diagnostic test would increase the feasibility of mass screening of dogs for infection and monitoring treatment efficacy in B. gibsoni‐infected dogs. Objective To determine the performance of a point‐of‐need insulated isothermal PCR (iiPCR) test for diagnosis of B. gibsoni in dogs rescued in dogfighting investigations. Animals Two hundred and thirty‐three dogs seized in dogfighting investigations. Methods Cross‐sectional study. Whole blood samples were tested for B. gibsoni and Babesia spp. by iiPCR. Results were compared to a reference standard comprised of concordant results from real‐time PCR in a commercial diagnostic laboratory and antibody titers. Results The iiPCR system was quick to learn, portable, and had a short processing time of |
Databáze: | OpenAIRE |
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