Microwave characterization of low-molecular-weight antioxidant specific biomarkers
| Autor: | Nickolay T. Cherpak, Alexander A. Barannik, A. I. Gubin, Natalia Naumova, Hanna Hlukhova, I. A. Protsenko, Svetlana Vitusevich |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Sodium ascorbate Antioxidant Resolution (mass spectrometry) medicine.medical_treatment Microfluidics Biophysics Dielectric Ascorbic Acid Biochemistry Antioxidants 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine medicine Electrochemistry Molecule Humans Computer Simulation Microwaves Molecular Biology chemistry.chemical_classification Reactive oxygen species Dielectric resonator Glutathione Molecular Weight Oxidative Stress 030104 developmental biology chemistry Linear Models Reactive Oxygen Species 030217 neurology & neurosurgery Biomarkers |
| Zdroj: | Biochimica et biophysica acta. General subjects. 1863(1) |
| ISSN: | 1872-8006 |
| Popis: | Antioxidants play a crucial role in the life sciences, as the regulators of biochemical reactions. We studied the dielectric properties of the low-molecular weight antioxidant specific biomarkers sodium ascorbate and glutathione in solutions of different concentrations. The biomarkers are multifunctional metabolites relevant to the reactive oxygen species (ROS) scavenging system of cells. The newly developed high-Q microwave whispering-gallery-mode (WGM) dielectric resonator based technique was applied. The technique allows investigation of liquids of nanoliter volumes filled in microfluidic channel within several milliseconds. The revealed peculiarities in the dependence of permittivity on concentrations of the sodium ascorbate and glutathione solutions are explained by differences in relaxation times and loses introduced by molecules of different shapes. We suggest that this novel approach offers the potential for the detection and characterization of ROS-relevant biomarkers with millisecond-time resolution. |
| Databáze: | OpenAIRE |
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