16S rRNA next generation sequencing analysis shows bacteria in Alzheimer’s post-mortem brain
| Autor: | Christy Waterfall, Maria Davies, Shelley J Allen, Deborah K. Shoemark, Nicola X West, Tanya L Cerajewska, David C. Emery, Thomas E Batstone, Jane A. Coghill |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Aging Pathology medicine.medical_specialty Cognitive Neuroscience Central nervous system Alzheimer’s disease (AD) microbiome next generation sequencing (NGS) Disease Biology DNA sequencing lcsh:RC321-571 03 medical and health sciences 0302 clinical medicine medicine Microbiome 16S rRNA bacteria lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry Neuroinflammation Original Research Bacteria Human microbiome human microbiome Ribosomal RNA biology.organism_classification 030104 developmental biology medicine.anatomical_structure Next generation sequencing (NGS) Alzheimer's disease (AD) 030217 neurology & neurosurgery Neuroscience |
| Zdroj: | Emery, D, Shoemark, D, Batstone, T, Waterfall, C, Coghill, J, Cerajewska, T, Davies, M, West, N & Allen-Birt, S 2017, ' 16S rRNA next generation sequencing analysis shows bacteria in Alzheimer’s post-mortem brain ', Frontiers in Aging Neuroscience, vol. 9, 195 . https://doi.org/10.3389/fnagi.2017.00195 Frontiers in Aging Neuroscience, Vol 9 (2017) Frontiers in Aging Neuroscience |
| DOI: | 10.3389/fnagi.2017.00195 |
| Popis: | The neurological deterioration associated with Alzheimer’s disease (AD), involving accumulation of amyloid-beta peptides and neurofibrillary tangles, is associated with evident neuroinflammation. This is now seen to be a significant contributor to pathology. Recently the tenet of the privileged status of the brain, regarding microbial compromise, has been questioned, particularly in terms of neurodegenerative diseases. It is now being considered that microbiological incursion into the central nervous system could be either an initiator or significant contributor to these. This is a novel study using 16S ribosomal gene-specific Next generation sequencing (NGS) of extracted brain tissue. Acomparison was made of the bacterial species content of both frozen and formaldehyde fixed sections of a small cohort of Alzheimer-affected cases with those of cognitively unimpaired (normal). Our findings suggest an increase in bacterial populations in Alzheimer brain tissue compared with normal. |
| Databáze: | OpenAIRE |
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