Replication-coupled histone H3.1 deposition determines nucleosome composition and heterochromatin dynamics during Arabidopsis seedling development
| Autor: | Céline Duc, Sophie Desset, Samuel Le Goff, Aline V. Probst, Sylviane Cotterell, Matthias Benoit, Lauriane Simon, Axel Poulet, Christophe Tatout |
|---|---|
| Přispěvatelé: | Génétique, Reproduction et Développement (GReD ), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Génétique, Reproduction et Développement (GReD), Centre National de la Recherche Scientifique (CNRS)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
DNA Replication
0106 biological sciences 0301 basic medicine Physiology Heterochromatin [SDV]Life Sciences [q-bio] Arabidopsis Plant Science Biology 01 natural sciences Histones 03 medical and health sciences Histone H3 Histone H1 Nucleosome [SDV.BV]Life Sciences [q-bio]/Vegetal Biology [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology Chromatin Assembly Factor-1 ComputingMilieux_MISCELLANEOUS Repetitive Sequences Nucleic Acid CAF-1 Arabidopsis Proteins Lysine [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biology Chromatin Assembly and Disassembly Plants Genetically Modified Nucleosomes Chromatin Cell biology 030104 developmental biology Histone Seedlings Mutation biology.protein RNA Splicing Factors 010606 plant biology & botany |
| Zdroj: | New Phytologist New Phytologist, Wiley, 2018, ⟨10.1111/nph.15248⟩ New Phytologist, 2018, ⟨10.1111/nph.15248⟩ |
| ISSN: | 0028-646X 1469-8137 |
| DOI: | 10.1111/nph.15248⟩ |
| Popis: | Developmental phase transitions are often characterized by changes in the chromatin landscape and heterochromatin reorganization. In Arabidopsis, clustering of repetitive heterochromatic loci into so-called chromocenters is an important determinant of chromosome organization in nuclear space. Here, we investigated the molecular mechanisms involved in chromocenter formation during the switch from a heterotrophic to a photosynthetically competent state during early seedling development. We characterized the spatial organization and chromatin features at centromeric and pericentromeric repeats and identified mutant contexts with impaired chromocenter formation. We find that clustering of repetitive DNA loci into chromocenters takes place in a precise temporal window and results in reinforced transcriptional repression. Although repetitive sequences are enriched in H3K9me2 and linker histone H1 before repeat clustering, chromocenter formation involves increasing enrichment in H3.1 as well as H2A.W histone variants, hallmarks of heterochromatin. These processes are severely affected in mutants impaired in replication-coupled histone assembly mediated by CHROMATIN ASSEMBLY FACTOR 1 (CAF-1). We further reveal that histone deposition by CAF-1 is required for efficient H3K9me2 enrichment at repetitive sequences during chromocenter formation. Taken together, we show that chromocenter assembly during post-germination development requires dynamic changes in nucleosome composition and histone post-translational modifications orchestrated by the replication-coupled H3.1 deposition machinery. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text