The Evaluation of a Low-Cost Colorimeter for Glucose Detection in Salivary Samples
| Autor: | Giovanny Chávez, Miguel A. Orozco, Rocio B. Dominguez, Alfredo Márquez-Lucero |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
Saliva
Analyte Calibration curve non-invasive 02 engineering and technology lcsh:Chemical technology 01 natural sciences Biochemistry Article Analytical Chemistry Limited access low-cost saliva RGB Diabetes Mellitus Humans lcsh:TP1-1185 Electrical and Electronic Engineering Instrumentation Detection limit Chemistry 010401 analytical chemistry Glucose detection Colorimeter 021001 nanoscience & nanotechnology Atomic and Molecular Physics and Optics 0104 chemical sciences Glucose Calibration Light emission 0210 nano-technology Biomedical engineering |
| Zdroj: | Sensors; Volume 17; Issue 11; Pages: 2495 Sensors (Basel, Switzerland) Sensors, Vol 17, Iss 11, p 2495 (2017) |
| ISSN: | 1424-8220 |
| DOI: | 10.3390/s17112495 |
| Popis: | Given the limited access to healthcare resources, low-income settings require the development of affordable technology. Here we present the design and evaluation of a low-cost colorimeter applied to the non-invasive monitoring of Diabetes Mellitus through the detection of glucose in salival fluid. Samples were processed by the glucose oxidase-peroxidase enzymatic system and analyzed with the development equipment. A light emission diode of 532.5 nm was used as an excitation source and a RGB module was used as a receptor. A calibration curve to quantify the concentration of salivary glucose (0 to 18 mg/dL) was carried out by relating the RGB components registered with glucose concentrations, achieving a limit of detection of 0.17 mg/dL with a CV of 5% (n = 3). Salivary samples of diabetic and healthy volunteers were processed with the equipment showing an average concentration of 1.5519 ± 0.4511 mg/dL for the first and 4.0479 ± 1.6103 mg/dL for the last, allowing a discrimination between both groups. Results were validated against a UV-Vis-NIR spectrophotometer with a correspondence of R2 of 0.98194 between both instruments. Results suggest the potential application of the developed device to the sensitive detection of relevant analytes with a low-cost, user-friendly, low-power and portable instrumentation. |
| Databáze: | OpenAIRE |
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