Dynamics of DNA during pulsed field electrophoresis in entangled and dilute polymer solutions
| Autor: | Xuelong. Shi, Michael D. Morris, Richard W. Hammond |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
chemistry.chemical_classification
Electrophoresis Quantitative Biology::Biomolecules Polymers Analytical chemistry Video microscopy Quantum entanglement Polymer Quantitative Biology::Genomics Random coil Analytical Chemistry Molecular dynamics chemistry.chemical_compound chemistry Microscopy Fluorescence Chemical physics Electric field DNA Fungal Hydroxyethyl cellulose |
| Zdroj: | Analytical chemistry. 67(18) |
| ISSN: | 0003-2700 |
| Popis: | Using fluorescence video microscopy, DNA electrophoretic behavior under field inversion conditions has been investigated in hydroxyethyl cellulose (HEC) solutions both above and below its entanglement limit. DNA conformational fluctuation periods are found to be strongly influenced by the frequency of the applied electric field. DNA maximum extension is found to be dependent on both the frequency and the strength of the applied field. It is proposed that both above and below the HEC entanglement limit, field inversion serves to keep the average DNA conformation in a size-dependent regime intermediate between full extension and random coil. In this time-averaged geometry, efficient long-chain DNA electrophoretic separation is enabled. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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