Validation of a new fluidic device for mechanical stimulation and characterization of microspheres: A first step towards cartilage characterization

Autor: E. Belamie, P. Valorge, Pascale Royer, Jade Berthelot, S. Devic, Noémie Petitjean, Danièle Noël, Patrick Cañadas, S. Le Floch, Christian Jorgensen, Marie Maumus, Gilles Dusfour
Přispěvatelé: Biomécanique des Interactions et de l'Organisation des Tissus et des Cellules (BIOTIC), Laboratoire de Mécanique et Génie Civil (LMGC), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Cellules Souches, Plasticité Cellulaire, Médecine Régénératrice et Immunothérapies (IRMB), Université de Montpellier (UM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier), Expérimentation & Calcul Scientifique (COMPEX), Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM ICMMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut de Chimie du CNRS (INC), École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL), CHU Montpellier, Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier), Gibier, François, Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM), Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Cartilage
Articular

Microsphere
Materials science
Characterization
Bioengineering
Stimulation
02 engineering and technology
010402 general chemistry
01 natural sciences
Biomaterials
Extracellular matrix
Chondrocytes
[SPI.MECA.BIOM] Engineering Sciences [physics]/Mechanics [physics.med-ph]/Biomechanics [physics.med-ph]
medicine
Device
Fluidics
Biomechanics
Reproducibility
Tissue Engineering
Cartilage
Reproducibility of Results
[SPI.MECA.BIOM]Engineering Sciences [physics]/Mechanics [physics.med-ph]/Biomechanics [physics.med-ph]
Mesenchymal Stem Cells
Repeatability
021001 nanoscience & nanotechnology
6. Clean water
Microspheres
0104 chemical sciences
Characterization (materials science)
medicine.anatomical_structure
Mechanics of Materials
0210 nano-technology
Chondrogenesis
Biomedical engineering
Zdroj: Materials Science and Engineering: C
Materials Science and Engineering: C, Elsevier, 2021, 121, pp.111800. ⟨10.1016/j.msec.2020.111800⟩
Materials Science and Engineering: C, 2021, 121, pp.111800. ⟨10.1016/j.msec.2020.111800⟩
ISSN: 0928-4931
DOI: 10.1016/j.msec.2020.111800⟩
Popis: International audience; Articular cartilage is made of chondrocytes surrounded by their extracellular matrix that can both sense and respond to various mechanical stimuli. One of the most widely used in vitro model to study cartilage growth is the model of mesenchymal stromal cells-derived cartilage micropellet. However, mechanical stimulation of micropellets has never been reported probably because of their small size and imperfect round shape. The objective of the study was to develop an original custom-made device allowing both the mechanical stimulation and characterization of cartilage micropellets. The fluidic-based device was designed for the concomitant stimulation or characterization of six microspheres placed into the conical wells of a tank. In the present study, the device was validated using alginate-, collagen- and crosslinked collagen-based microspheres. Different types and ranges of pressure signals (square, sinusoidal and constant) were applied. The mechanical properties of microspheres were equivalent to those determined by a conventional compression test. Accuracy, repeatability and reproducibility of all types of pressure signals were demonstrated even though square signals were less accurate and sinusoidal signals were less reproducible than the others. The interest of this new device lies in the reliability to mechanically stimulate and characterize microspheres with diameters in the range of 900 to 1500 μm. Mechanical stimulation can be performed on six microspheres in parallel allowing the mechanical and molecular characterization of the same group of cartilage micropellets. The device will be useful to evaluate the growth of cartilage micropellets under mechanical stimuli.
Databáze: OpenAIRE