Identification of homogeneously staining regions by G-banding and chromosome microdissection, and FISH marker selection using human Alu sequence primers in a scleractinian coral Coelastrea aspera Verrill, 1866 (Cnidaria)
| Autor: | Takahiro Taguchi, Erika Tagami, Satoko Sekida, Satoshi Kubota, Kazuo Okuda, Shu Nakachi, Takuma Mezaki, Akira Tominaga |
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| Rok vydání: | 2015 |
| Předmět: |
0106 biological sciences
0301 basic medicine G banding ved/biology.organism_classification_rank.species Alu element Plant Science Biology 010603 evolutionary biology 01 natural sciences Alu repeats 03 medical and health sciences Cnidaria FISH Genetics medicine Animalia Ribosomal DNA Research Articles medicine.diagnostic_test ved/biology fungi Coelastrea aspera Ribosomal RNA Chromosome microdissection karyotype 030104 developmental biology Animal Science and Zoology Coral Nucleolus organizer region hsr chromosome microdissection Biotechnology Fluorescence in situ hybridization |
| Zdroj: | Comparative Cytogenetics Comparative Cytogenetics 10(1): 61-75 |
| ISSN: | 1993-0771 |
| Popis: | Karyotype analysis was performed on the scleractinian coral Coelastrea aspera Verrill, 1866, commonly found along temperate coasts in Japan (30–35°N) and in coastal waters in the Indian and Pacific oceans. G-banding of C. aspera was successfully performed, although the banding pattern was not as clear as that in mammals. The karyogram clearly revealed that this coral had a homogeneously staining region (hsr) in chromosome 11. This hsr consisted of ribosomal RNA (rRNA) related genes, which was demonstrated by fluorescence in situ hybridization (FISH) with probes generated using 28S ribosomal DNA (rDNA) primers and those generated through chromosome microdissection. In addition, we conducted silver-stained nucleolus organizer region (Ag-NOR) analysis and found Ag depositions in the interphase nuclei but not on rRNA gene loci and hsr(s) in the mitotic stage. The hsr of this coral was observed in approximately 50% of the metaphase spreads analyzed. This may explain the diversity of coral rDNA based on the molecular study of sequence analysis. Furthermore, it was discovered that human telomere and Alu repeated sequences were present in this C. aspera. Probes derived from human Alu sequences are expected to play an important role in the classification of corals. Overall, our data can be of great value in discriminating among scleractinian coral species and understanding their genetics, including chromosomal evolution. |
| Databáze: | OpenAIRE |
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