Elevated UMOD methylation level in peripheral blood is associated with gout risk
| Autor: | Yuting Jiang, Yiyi Mao, Jie Dai, Haochang Hu, Hang Yu, Xiaoying Chen, Yong Yang, Shiwei Duan |
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| Rok vydání: | 2017 |
| Předmět: |
Adult
Male musculoskeletal diseases 0301 basic medicine medicine.medical_specialty Tamm–Horsfall protein Gout lcsh:Medicine Real-Time Polymerase Chain Reaction Risk Assessment Sensitivity and Specificity Article 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Internal medicine Uromodulin Gene expression medicine Humans lcsh:Science Aged 030203 arthritis & rheumatology Genetics Multidisciplinary biology lcsh:R Methylation DNA Methylation Middle Aged medicine.disease Uric Acid 030104 developmental biology Real-time polymerase chain reaction Endocrinology chemistry CpG site DNA methylation biology.protein Uric acid lcsh:Q Blood Chemical Analysis |
| Zdroj: | Scientific Reports, Vol 7, Iss 1, Pp 1-7 (2017) Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-017-11627-w |
| Popis: | Uromodulin (UMOD) encodes an uromodulin glycoprotein, and its mutation results in uromodulin glycoprotein dysfunction and the occurrence of gout. The aim of our study was to assess whether UMOD methylation could predict the risk of gout. A total of 89 sporadic gout cases and 103 age and gender-matched healthy controls were recruited in this study. UMOD methylation level was determined by quantitative methylation-specific PCR (qMSP) in peripheral blood, and the percentage of methylated reference (PMR) was described to represent the methylation level. Our results showed that UMOD methylation was significantly higher in gout cases than controls (median: 1.45 versus 0.75, P UMOD methylation in gout was 0.764 (P = 2.90E-10) with a sensitivity of 65.2% and a specificity of 88.3%. UMOD methylation level was shown to be significantly correlated with the serum level of uric acid (UA) (r = −0.208, P = 0.035). Besides, the luciferase reporter assay showed that UMOD CpG island region was able to upregulate gene expression (fold change = 2, P = 0.004). In conclusion, UMOD methylation assessment might be used to predict the occurrence of gout. |
| Databáze: | OpenAIRE |
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