Scanningless and continuous 3D bioprinting of human tissues with decellularized extracellular matrix
| Autor: | Claire Yu, Wei Zhu, Jacob Stupin, Shaochen Chen, Alexandria Hairabedian, Pengrui Wang, Kathleen L. Miller, Anna Koroleva-Maharajh, Xuanyi Ma |
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| Rok vydání: | 2019 |
| Předmět: |
Swine
Induced Pluripotent Stem Cells Biophysics Bioengineering 02 engineering and technology Regenerative medicine Article law.invention Biomaterials Extracellular matrix 03 medical and health sciences law Extracellular Animals Humans Myocytes Cardiac Human Induced Pluripotent Stem Cells Induced pluripotent stem cell 030304 developmental biology 0303 health sciences 3D bioprinting Decellularization Tissue Engineering Tissue Scaffolds Bioprinting 021001 nanoscience & nanotechnology Extracellular Matrix Cell biology Mechanics of Materials Printing Three-Dimensional Hepatocytes Ceramics and Composites 0210 nano-technology |
| Zdroj: | Biomaterials. 194:1-13 |
| ISSN: | 0142-9612 |
| DOI: | 10.1016/j.biomaterials.2018.12.009 |
| Popis: | Decellularized extracellular matrices (dECMs) have demonstrated excellent utility as bioscaffolds in recapitulating the complex biochemical microenvironment, however, their use as bioinks in 3D bioprinting to generate functional biomimetic tissues has been limited by their printability and lack of tunable physical properties. Here, we describe a method to produce photocrosslinkable tissue-specific dECM bioinks for fabricating patient-specific tissues with high control over complex microarchitecture and mechanical properties using a digital light processing (DLP)-based scanningless and continuous 3D bioprinter. We demonstrated that tissue-matched dECM bioinks provided a conducive environment for maintaining high viability and maturation of human induced pluripotent stem cell (hiPSC)-derived cardiomyocytes and hepatocytes. Microscale patterning also guided spontaneous cellular reorganization into predesigned striated heart and lobular liver structures through biophysical cues. Our methodology enables a light-based approach to rapidly bioprint dECM bioinks with accurate tissue-scale design to engineer physiologically-relevant functional human tissues for applications in biology, regenerative medicine, and diagnostics. |
| Databáze: | OpenAIRE |
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