Effects of a membrane-metallopeptidase blocking agent thiorphan in long-term cultures of human bone marrow
| Autor: | Mirna Sučić, Branka Golubić-Ćepulić, Milivoj Boranić, Boris Labar, Igor Aurer, Silvana Stanović Janda, Mladen Petrovečki |
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| Rok vydání: | 2000 |
| Předmět: |
Thiorphan
Stromal cell Metallopeptidase Cell Bone Marrow Cells Pharmacology chemistry.chemical_compound Cytology medicine Humans Protease Inhibitors Clonogenic assay Cells Cultured biology business.industry Calla Cell Differentiation Hematology biology.organism_classification Haematopoiesis medicine.anatomical_structure chemistry Immunology Neprilysin Long-term bone marrow culture (LTBMC) GM-CFU macrophages Membrane metalloendopeptidase (CD10 EC 3.4.24.11) thiorphan ovarian cancer business Cell Division |
| Zdroj: | Haematologia. 30:289-302 |
| ISSN: | 1568-5594 0017-6559 |
| DOI: | 10.1163/156855900300109530 |
| Popis: | Thiorphan [(DL-3-mercapto-2-benzylpropanoyl)-glycine], a drug blocking the activity of membrane metalloendopeptidase EC 3.4.24.11 (CD10, CALLA), was added to long-term cultures of human bone marrow. Progression of the cultures was assessed by cell counts, cytology and clonogenic (GM-CFU) ability of the non-adherent cells in the supernatant and by morphology of the adherent stromal layer. A stimulatory effect on hematopoiesis was noted. |
| Databáze: | OpenAIRE |
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