Two Families with Familial Amyotrophic Lateral Sclerosis Are Linked to a Novel Locus on Chromosome 16q
| Autor: | Vianney de Jong, Frank Baas, Deborah Ruddy, Christopher Shaw, John Powell, M J Parton, Cathryn M. Lewis, Ammar Al-Chalabi, Teepu Siddique, P. Nigel Leigh, Caroline Vance, Bradley N. Smith, Eelco Postumus Meyjes |
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| Přispěvatelé: | Faculteit der Geneeskunde, Genome Analysis |
| Rok vydání: | 2003 |
| Předmět: |
Adult
Male Candidate gene Locus (genetics) Biology 03 medical and health sciences 0302 clinical medicine Chromosome 16 Superoxide Dismutase-1 Genetic linkage Report medicine Genetics Humans Genetics(clinical) Amyotrophic lateral sclerosis Genetics (clinical) 030304 developmental biology Aged 0303 health sciences Super oxide dismutase Superoxide Dismutase Haplotype Amyotrophic Lateral Sclerosis Chromosome Mapping Middle Aged medicine.disease Penetrance 3. Good health Pedigree Europe Haplotypes Female Lod Score 030217 neurology & neurosurgery Chromosomes Human Pair 16 Microsatellite Repeats |
| Zdroj: | American Journal of Human Genetics, 73, 390-396. Cell Press American journal of human genetics, 73(2), 390-396. Cell Press |
| ISSN: | 0002-9297 |
| DOI: | 10.1086/377157 |
| Popis: | Amyotrophic lateral sclerosis (ALS) is a fatal adult-onset disease in which motor neurons in the brain and spinal cord degenerate by largely unknown mechanisms. ALS is familial (FALS) in 10% of cases, and the inheritance is usually dominant, with variable penetrance. Mutations in copper/zinc super oxide dismutase (SOD1) are found in 20% of familial and 3% of sporadic ALS cases. Five families with ALS and frontotemporal dementia (ALS-FTD) are linked to 9q21, whereas one family with pure ALS is linked to 18q21. We identified two large European families with ALS without SOD1 mutations or linkage to known FALS loci and conducted a genomewide linkage screen using 400 microsatellite markers. In both families, two-point LOD scores1 and a haplotype segregating with disease were demonstrated only across regions of chromosome 16. Subsequent fine mapping in family 1 gave a maximum two-point LOD score of 3.62 at D16S3137 and a three-point LOD score of 3.85 for markers D16S415 and D16S3137. Haplotype analysis revealed no recombinationapproximately 30 cM, (flanking markers at D16S3075 and D16S3112). The maximum two-point LOD score for family 2 was 1.84 at D16S415, and the three-point LOD score was 2.10 for markers D16S419 and D16S415. Definite recombination occurred in several individuals, which narrowed the shared haplotype in affected individuals to a 10.1-cM region (flanking markers: D16S3396 and D16S3112). The region shared by both families on chromosome 16q12 corresponds to approximately 4.5 Mb on the Marshfield map. Bioinformatic analysis of the region has identified 18 known genes and 70 predicted genes in this region, and sequencing of candidate genes has now begun. |
| Databáze: | OpenAIRE |
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