Fabrication and biological properties of calcium phosphate/chitosan composite coating on titanium in modified SBF
| Autor: | Young-Hwa Jeong, Hyun-Joo Moon, Yeong-Joon Park, Seok-Jae Kim, Kyung-Hee Park, Ho-Jun Song |
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| Rok vydání: | 2018 |
| Předmět: |
Calcium Phosphates
0301 basic medicine Materials science Biocompatibility Simulated body fluid Composite number chemistry.chemical_element Bioengineering 02 engineering and technology Apatite Cell Line Nanocomposites Biomaterials Chitosan 03 medical and health sciences chemistry.chemical_compound Cell Adhesion Humans Substrate (chemistry) 021001 nanoscience & nanotechnology Body Fluids Nanostructures 030104 developmental biology chemistry Chemical engineering Mechanics of Materials visual_art Microscopy Electron Scanning visual_art.visual_art_medium 0210 nano-technology Mesoporous material Titanium |
| Zdroj: | Materials Science and Engineering: C. 90:113-118 |
| ISSN: | 0928-4931 |
| DOI: | 10.1016/j.msec.2018.04.060 |
| Popis: | In order to increase the biocompatibility and bioactivity of chitosan, hydroxyapatite was in situ combined into the spin-coated chitosan layer on the titanium substrate by incubating in modified simulated body fluid (m-SBF). The calcium phosphate/chitosan (CaP/CS) composite prepared in m-SBF showed a homogeneous distribution of spherical nano-clusters. The hydrophilicity of the coatings was increased by performing NaOH post-treatment of CaP/CS composites, which also affected apatite formation. Biocompatibility of the coatings was assessed by investigating the cellular response of human osteoblast-like MG-63 cells with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Cell adhesion and osteogenic properties of the mesoporous CaP/CS composite were evaluated by SEM and ALPase assay, respectively. This in vitro study showed improved cell adhesion and differentiation on nanostructured CaP/CS composites. These results indicate that this CaP/CS composite could be a promising candidate for bone tissue engineering. |
| Databáze: | OpenAIRE |
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