Diverse human extracellular RNAs are widely detected in human plasma
| Autor: | Fabio C. P. Navarro, Raymond Y. Kwong, Robert R. Kitchen, Yaoyu E. Wang, Kirsty Danielson, Ravi V. Shah, Lea M. Beaulieu, Timur R. Galeev, Saumya Das, Venkatesh L. Murthy, Mark Gerstein, Selim E. Tanriverdi, Jane E. Freedman, Daniel Levy, Kahraman Tanriverdi, Eric Mick, Joel Rozowsky, Alex Holman, Milka Koupenova-Zamor, Ekaterina Mikhalev |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Genetics Regulation of gene expression Multidisciplinary Science General Physics and Astronomy RNA Piwi-interacting RNA General Chemistry Biology General Biochemistry Genetics and Molecular Biology Long non-coding RNA 03 medical and health sciences 030104 developmental biology microRNA Human genome Small nucleolar RNA Gene |
| Zdroj: | Nature Communications, Vol 7, Iss 1, Pp 1-14 (2016) |
| ISSN: | 2041-1723 |
| Popis: | There is growing appreciation for the importance of non-protein-coding genes in development and disease. Although much is known about microRNAs, limitations in bioinformatic analyses of RNA sequencing have precluded broad assessment of other forms of small-RNAs in humans. By analysing sequencing data from plasma-derived RNA from 40 individuals, here we identified over a thousand human extracellular RNAs including microRNAs, piwi-interacting RNA (piRNA), and small nucleolar RNAs. Using a targeted quantitative PCR with reverse transcription approach in an additional 2,763 individuals, we characterized almost 500 of the most abundant extracellular transcripts including microRNAs, piRNAs and small nucleolar RNAs. The presence in plasma of many non-microRNA small-RNAs was confirmed in an independent cohort. We present comprehensive data to demonstrate the broad and consistent detection of diverse classes of circulating non-cellular small-RNAs from a large population. |
| Databáze: | OpenAIRE |
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