Immunologically cross-reactive and redox-competent cytochrome b6/f-complexes in the chlorophyll-free plasma membrane of cyanobacteria
| Autor: | Marnik Wastyn, Günter A. Peschek, Harald Kraushaar, Silvia Hager |
|---|---|
| Jazyk: | angličtina |
| Předmět: |
Chlorophyll
Inhibitor Biophysics Respiratory chain Cross Reactions Cyanobacteria Biochemistry Cytochrome-c reductase Thylakoid membrane Electron Transport Species Specificity Structural Biology F-ATPase Genetics Molecular Biology Cytochrome f biology Cell Membrane Synechocystis food and beverages Cell Biology NAD biology.organism_classification Cytochromes f Molecular Weight Chloroplast Kinetics Membrane Thylakoid Cytochromes Oxidation-Reduction Anabaena variabilis Cytochrome b6/f-complex Plasma membrane |
| Zdroj: | FEBS Letters. (1-2):227-231 |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/0014-5793(90)81091-2 |
| Popis: | Plasma and thylakoid membranes were separated and purified from cell-free extracts of the cyanobacteria Anacystis nidulans, Synechocystis 6714, Anabaena variabilis and Nostoc sp. strain Mac. Immunoblots of the membrane proteins using antisera raised against subunits I–IV of the chloroplast b6/f-complex gave evidence for the presence of a homologous complex in both plasma and thylakoid membranes from the four species of cyanobacteria investigated. Both plasma and thylakoid membranes catalyzed the electron transfer from (exogenous) plastoquinol-9 and NADH to horse heart ferricytochrome c. However, while with plasma membranes these reactions were severely inhibited by low concentrations of antimycin A and rotenone, respectively, the inhibitors were without major effect on thylakoid membranes. The results will be discussed in terms of a possible similarity (analogy and/or homology?) of cyanobacterial plasma membranes to the inner mitochondrial membrane. |
| Databáze: | OpenAIRE |
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