Complete Genomic Sequence of Bacteriophage H188: A Novel Vibrio kanaloae Phage Isolated from Yellow Sea
| Autor: | Xue Song, Yan Li, Yong Jiang, Duobing Wang, Min Wang, Qian Liu, Hongbing Shao, Yu Ma |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Oceans and Seas viruses 030106 microbiology Genome Viral Applied Microbiology and Biotechnology Microbiology Genome Bacteriophage Open Reading Frames Viral Proteins 03 medical and health sciences Bacteriophages Seawater Genome size Phylogeny Vibrio Sequence (medicine) Vibrio kanaloae Genetics Base Composition biology DNA replication Genomics General Medicine biology.organism_classification Molecular biology Titer Open reading frame 030104 developmental biology Myoviridae |
| Zdroj: | Current Microbiology. 72:628-633 |
| ISSN: | 1432-0991 0343-8651 |
| DOI: | 10.1007/s00284-015-0984-6 |
| Popis: | Phage H188, a novel Vibrio kanaloae phage, was isolated from the surface water of Yellow Sea. Morphological analysis by transmission electron microscopy reveals that it belongs to the family Myoviridae. Present result suggests that the phage is stable at pH between 4.0 and 12.0. No significant difference in phage titers is noted at temperature 30-50 °C. A latent period of approximately 96 mins is indicated by the one-step growth curve. And, the burst size is about three virions per cell. Furthermore, genomic analysis of H188 reveals a genome size of 50364 bp with 43.63 % G+C content, and 76 putative open reading frames. There is no obvious similarity between H188 and other known phages by genomic comparison. Moreover, the H188 genome includes modules for phage structure, phage packaging, DNA replication and regulation, and some additional functions. |
| Databáze: | OpenAIRE |
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