Replication of human immunodeficiency virus in HL-60 cells
| Autor: | Claude Jasmin, M. Semmel, José J. Aguilar, A. Alileche, Vladimir A. Morozov, S. Plaisance, A. Macho, D. Coulaud |
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| Předmět: |
viruses
T-Lymphocytes Immunology Molecular Sequence Data Biology Gp41 Virus Replication Virus Cell Line Viral Proteins Antigen Virology Humans chemistry.chemical_classification Syncytium Base Sequence Virion virus diseases HIV RNA-Directed DNA Polymerase Antigens Differentiation Microscopy Electron Viral replication chemistry Cell culture CD4 Antigens Ultrastructure Glycoprotein |
| Zdroj: | Europe PubMed Central |
| Popis: | HL-60 cells carrying the CD4 marker could be productively infected with human immunodeficiency virus (HIV) but did not form syncytia, though 11-14 days p.i., there was a transient decrease in cell multiplication and viability. After prolonged passage, a subpopulation of HL-60 cells was selected. The virus produced differed from the initial input virus grown on CEM cells: the virions lacked knobs, were either empty or had abnormal cores, had a higher ratio p24/gp41,gp110 and were less infectious. After prolonged passage, virus was produced which was fully infectious for CEM but not for fresh HL-60 cells, and which ressembled the input virus with respect to morphology and p24/gp41,gp110 ratio. |
| Databáze: | OpenAIRE |
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