Detection of Beijing strains of MDR M. tuberculosis and their association with drug resistance mutations in katG, rpoB, and embB genes

Autor: Pallavi Sinha, Pramod Kumar Gupta, Nalin Rastogi, Savita Kulkarni, Shampa Anupurba, Vijay Nema, Pampi Chakraborty, Anamika Gupta
Přispěvatelé: Banaras Hindu University [Varanasi] (BHU), National AIDS Research Institute, Bhabha Atomic Research Centre (BARC), Government of India, Department of Atomic Energy, Unité de la Tuberculose et des Mycobactéries - WHO Supranational TB Reference Laboratory, Institut Pasteur de la Guadeloupe, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Organisation Mondiale de la Santé / World Health Organization Office (OMS / WHO), Institute of Medical Sciences [BHU, Vanarasi] (IMS-BHU), This study was financially supported by the Indian Council of Medical Research, New Delhi. The funders had no role in study design, data collection, and analysis, decision to publish, or preparation of the manuscript., Financial assistance to Dr. Anamika Gupta from the Indian Council of Medical Research is also highly acknowledged.
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Male
0301 basic medicine
MESH: Mycobacterium tuberculosis
MESH: Mutation Rate
Antitubercular Agents
Drug resistance
MESH: Genotype
MESH: Aged
80 and over
Mutation Rate
Beijing
Drug Resistance
Multiple
Bacterial
MESH: Child
Tuberculosis
Multidrug-Resistant
Genotype
Child
MESH: Bacterial Proteins
MESH: Pentosyltransferases
Aged
80 and over
Spoligotyping
MESH: Aged
MESH: Microbial Sensitivity Tests
MESH: Middle Aged
biology
MESH: Multiplex Polymerase Chain Reaction
Drug resistant-TB
DNA-Directed RNA Polymerases
Middle Aged
Catalase
3. Good health
Infectious Diseases
MESH: Young Adult
Female
Research Article
Adult
Tuberculosis
Adolescent
030106 microbiology
India
Beijing genotype
Microbial Sensitivity Tests
lcsh:Infectious and parasitic diseases
Mycobacterium tuberculosis
Young Adult
03 medical and health sciences
Bacterial Proteins
MESH: Catalase
medicine
Humans
Transmission
lcsh:RC109-216
Pentosyltransferases
MESH: Tuberculosis
Multidrug-Resistant
Aged
MESH: Adolescent
MESH: Humans
MESH: Adult
Rv2820
MESH: Drug Resistance
Multiple
Bacterial
rpoB
medicine.disease
biology.organism_classification
Virology
MESH: Antitubercular Agents
[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology
MESH: Male
MESH: DNA-Directed RNA Polymerases
Multiple drug resistance
030104 developmental biology
Parasitology
MESH: India
Multiplex Polymerase Chain Reaction
MESH: Female
Zdroj: BMC Infectious Diseases
BMC Infectious Diseases, BioMed Central, 2020, 20 (1), pp.752. ⟨10.1186/s12879-020-05479-5⟩
BMC Infectious Diseases, Vol 20, Iss 1, Pp 1-7 (2020)
ISSN: 1471-2334
DOI: 10.1186/s12879-020-05479-5⟩
Popis: Background Molecular epidemiological studies of Mycobacterium tuberculosis (MTB) are the core of current research to find out the association of the M. tuberculosis genotypes with its outbreak and transmission. The high prevalence of the Beijing genotype strain among multidrug resistance (MDR) TB has already been reported in various studies around India. The overall objective of this study was to detect the prevalence of Beijing genotype strains of MDR M. tuberculosis and their association with the clinical characteristics of TB patients. Methods In this study 381 M. tuberculosis clinical isolates were obtained from sputum samples from 2008 to 2014. The multiplex-PCR and Spoligotyping (n = 131) methods were used to investigate the prevalence of the Beijing genotype strain by targeting the Rv2820 gene and their association with drug resistance and clinical characteristics of TB patients. The drug susceptibility testing of first-line anti-TB drugs was performed by using the proportion method and MGIT960. A collection of isolates having Beijing and non-Beijing strains were also characterized to see if Beijing genotype strains had a higher rate of mutations at codons 516, 526 and 531 of the 81-bp region of the rpoB gene, codon 315 of the katG gene, and codon 306 of the embB gene. Results The sensitivities and specificities of multiplex-PCR assay compared to that of standard Spoligotyping was detected to be 100%. Further, we observe that the multi drug-resistance was significantly associated with Beijing genotype strains (p = 0.03) and a strong correlation between Beijing genotype strains and specific resistance mutations at the katG315, rpoB531, and embB306 codons (p = Conclusions This rapid, simple, and cost-effective multiplex PCR assay can effectively be used for monitoring the prevalence of Beijing genotype strains in low resource settings. Findings of this study may provide a scientific basis for the development of new diagnostic tools for detection and effective management of DR-TB in countries with a higher incidence rate of Beijing genotype strains.
Databáze: OpenAIRE
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