Designer Extracellular Matrix Based on DNA-Peptide Networks Generated by Polymerase Chain Reaction
| Autor: | Andreas Marx, Holger Bußkamp, Marilena Manea, Alexander Finke |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Cell type
Integrin Molecular Conformation Peptide 02 engineering and technology 010402 general chemistry Polymerase Chain Reaction 01 natural sciences Catalysis Extracellular matrix chemistry.chemical_compound Tissue engineering Cell Line Tumor Humans Cell adhesion chemistry.chemical_classification biology DNA General Chemistry 021001 nanoscience & nanotechnology Cyclic peptide Extracellular Matrix 0104 chemical sciences HEK293 Cells chemistry ddc:540 Biophysics biology.protein Peptides 0210 nano-technology HeLa Cells |
| Zdroj: | Angewandte Chemie International Edition. 55:10136-10140 |
| ISSN: | 1433-7851 |
| DOI: | 10.1002/anie.201604687 |
| Popis: | Cell proliferation and differentiation in multicellular organisms are partially regulated by signaling from the extracellular matrix. The ability to mimic an extracellular matrix would allow particular cell types to be specifically recognized, which is central to tissue engineering. We present a new functional DNA-based material with cell-adhesion properties. It is generated by using covalently branched DNA as primers in PCR. These primers were functionalized by click chemistry with the cyclic peptide c(RGDfK), a peptide that is known to predominantly bind to αvβ3 integrins, which are found on endothelial cells and fibroblasts, for example. As a covalent coating of surfaces, this DNA-based material shows cell-repellent properties in its unfunctionalized state and gains adhesiveness towards specific target cells when functionalized with c(RGDfK). These cells remain viable and can be released under mild conditions by DNase I treatment. published |
| Databáze: | OpenAIRE |
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