Deciphering influences of testosterone and dihydrotestosterone on lipid metabolism genes using brown trout primary hepatocytes
| Autor: | Eduardo Rocha, Tânia Vieira Madureira, Célia Lopes, Inês L. Pereira |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
endocrine system
medicine.medical_specialty Trout Health Toxicology and Mutagenesis 010501 environmental sciences Aquatic Science urologic and male genital diseases Fatty Acid-Binding Proteins 01 natural sciences 03 medical and health sciences Internal medicine medicine Animals Testosterone 030304 developmental biology 0105 earth and related environmental sciences chemistry.chemical_classification 0303 health sciences Lipogenesis Fatty Acids Fatty acid Lipid metabolism Dihydrotestosterone Peroxisome Lipid Metabolism PPAR gamma Endocrinology chemistry Liver Adipogenesis Androgens Hepatocytes lipids (amino acids peptides and proteins) Water Pollutants Chemical Lipoprotein medicine.drug |
| Zdroj: | Aquatic toxicology (Amsterdam, Netherlands). 235 |
| ISSN: | 1879-1514 |
| Popis: | Despite of physiological and toxicological relevance, the potential of androgens to influence fish lipid metabolism remains poorly explored. Here, brown trout primary hepatocytes were exposed to six concentrations (1 nM to 100 μM) of dihydrotestosterone (DHT) and testosterone (T), to assess changes in the mRNA levels of genes covering diverse lipid metabolic pathways. Acsl1, essential for fatty acid activation, was up-regulated by T and DHT, whereas the lipogenic enzymes FAS and ACC were up-regulated by the highest (100 μM) concentration of T and DHT, respectively. ApoA1, the major component of high-density lipoprotein (HDL), was down-regulated by both androgens. PPARγ, linked to adipogenesis and peroxisomal β-oxidation, was down-regulated by T and DHT, while Acox1–3I, rate-limiting in peroxisomal β-oxidation, was down-regulated by T. Fabp1, StAR and LPL were not altered. Our findings suggest that androgens may impact on lipid transport, adipogenesis and fatty acid β-oxidation and promote lipogenesis in fish liver. |
| Databáze: | OpenAIRE |
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