Antibody-mediated inhibition of Nogo-A signaling promotes neurite growth in PC-12 cells
| Autor: | Ennio Tasciotti, Roberto Palomba, Shilpa Scaria, Alvaro Muñoz, Sarah Hmaidan, Nima Taghipour, Iman K. Yazdi, Timothy B. Boone |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Neurofilament Neurite medicine.drug_class Cell Biomedical Engineering Medicine (miscellaneous) Monoclonal antibody Biomaterials lcsh:Biochemistry 03 medical and health sciences 0302 clinical medicine mental disorders medicine lcsh:QD415-436 Viability assay neuroregeneration biology Neuroregeneration Molecular biology Cell biology Tissue Engineering and Regenerative Medicine: Research from Houston Methodist Research Institute 030104 developmental biology medicine.anatomical_structure Cell culture bioactivity biology.protein Antibody Nogo-A 030217 neurology & neurosurgery PC-12 cells psychological phenomena and processes |
| Zdroj: | Journal of Tissue Engineering, Vol 7 (2016) Journal of Tissue Engineering |
| ISSN: | 2041-7314 |
| Popis: | The use of a monoclonal antibody to block the neurite outgrowth inhibitor Nogo-A has been of great interest for promoting axonal recovery as a treatment for spinal cord injury. While several cellular and non-cellular assays have been developed to quantify the bioactive effects of Nogo-A signaling, demand still exists for the development of a reliable approach to characterize the effectiveness of the anti-Nogo-A antibody. In this study, we developed and validated a novel cell-based approach to facilitate the biological quantification of a Nogo-A antibody using PC-12 cells as an in vitro neuronal cell model. Changes in the mRNA levels of the neuronal differentiation markers, growth-associated protein 43 and neurofilament light-polypeptide, suggest that activation of the Nogo-A pathway suppresses axonal growth and dendrite formation in the tested cell line. We found that application of anti-Nogo-A monoclonal antibody can significantly enhance the neuronal maturity of PC-12 cells by blocking the Nogo-A inhibitory effects, providing enhanced effects on neural maturity at the molecular level. No adverse effects were observed on cell viability. |
| Databáze: | OpenAIRE |
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