Efficacy of free and encapsulated Bacillus lichenformis strain SL10 on degradation of phenol: A comparative study of degradation kinetics
| Autor: | A. Gnanamani, R. Thilagam, S. Chris Felshia, N. Aswin Karthick, K.S.M.S. Raghavarao, A. Chandralekha |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Whey protein Environmental Engineering Bacillus 010501 environmental sciences Management Monitoring Policy and Law 01 natural sciences Microbiology 03 medical and health sciences chemistry.chemical_compound Dioxygenase Phenol Bacillus licheniformis Waste Management and Disposal 0105 earth and related environmental sciences Pollutant Catechol Chromatography Sewage biology General Medicine biology.organism_classification Soil contamination Kinetics Biodegradation Environmental 030104 developmental biology chemistry Wastewater |
| Zdroj: | Journal of Environmental Management. 197:373-383 |
| ISSN: | 0301-4797 |
| Popis: | The present study exemplifies phenol degradation efficacy of the free and encapsulated bacterial isolate, explored the degradation kinetics and storage stability in detail. In brief, isolation, identification and phenol degradation potential of the bacterial made from wastewater treated sludge samples. The organism identified as B. licheniformis demonstrates phenol degradation at a concentration more than 1500 ppm. Optimization of environmental parameters reduces the time taken for degradation considerably. The organism has further been encapsulated using whey protein and the efficacy of encapsulated species suggested that encapsulation protects the cells from high concentration of phenol and at the same time expedite the degradation of the chosen pollutant at appreciable level. The encapsulated species effectively degrade 3000 ppm concentration of phenol within 96 h of incubation. Both pH and temperature stability observed in the encapsulated species suggests the effectiveness of the encapsulation. The encapsulated cells displayed storage stability for a four week period at 4 C and reusability up to three exposures. Degradation effected through intracellular catechol 2,3 dioxygenase. In conclusion, encapsulation of B. licheniformis (i) protects the cells from direct exposure to toxic pollutants; (ii) facilitates the field scale application and (iii) eliminate the practical difficulties in handling wet biomass in field application and assures the best possible way of remediating the phenol contaminated soil. |
| Databáze: | OpenAIRE |
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